1H-NMR and LC-MS Based Metabolomics Analysis of Potato (Solanum tuberosum L.) Cultivars Irrigated with Fly Ash Treated Acid Mine Drainage

被引：6

作者：

Raletsena, Maropeng V. ^{[1
]}

Mdlalose, Samukelisiwe ^{[1
]}

Bodede, Olusola S. ^{[1
]}

Assress, Hailemariam A. ^{[2
,3
]}

Woldesemayat, Adugna A. ^{[4
]}

Modise, David M. ^{[5
]}

机构：

[1] Coll Agr & Environm Sci, Dept Agr & Anim Hlth, Private Bag X6, ZA-1710 Florida, South Africa

[2] Arkansas Childrens Nutr Ctr, 15 Childrens Way, Little Rock, AR 72202 USA

[3] Univ Arkansas Med Sci, Dept Pediat, Little Rock, AR 72202 USA

[4] Addis Ababa Sci & Technol Univ, Dept Biotechnol, Genom & Bioinformat Res Unit, POB 16417, Addis Ababa, Ethiopia

[5] North West Univ, Fac Nat & Agr Sci, Food Secur & Safety FSS, Private Bag X2046, ZA-2735 Mahikeng, South Africa

来源：

MOLECULES | 2022年 / 27卷 / 04期

关键词：

acid mine drainage (AMD); fly ash (FA); metabolites; metabolomics; nuclear magnetic resonance (NMR); potato (Solanum tuberosum L; CHLOROGENIC ACID; SOIL; STRESS; NMR; SOLANIDINE; RESISTANCE; REMOVAL; QUALITY; IMPACT; SPECTROSCOPY;

D O I：

10.3390/molecules27041187

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

H-1 NMR and LC-MS, commonly used metabolomics analytical platforms, were used to annotate the metabolites found in potato (Solanum tuberosum L.) irrigated with four different treatments based on FA to AMD ratios, namely: control (0% AMD; tap water), 1:1 (50% AMD), 3:1 (75% AMD is 75% FA: AMD), and 100% AMD (untreated). The effects of stress on plants were illustrated by the primary metabolite shifts in the region from delta(H) 0.0 to delta(H) 4.0 and secondary metabolites peaks were prominent in the region ranging from delta(H) 4.5 to delta(H) 8.0. The 1:3 irrigation treatment enabled, in two potato cultivars, the production of significantly high concentrations of secondary metabolites due to the 75% FA: AMD content in the irrigation mixture, which induced stress. The findings suggested that 1:1 irrigation treatment induced production of lower amounts of secondary metabolites in all crops compared to crops irrigated with untreated acid mine drainage treatment and with other FA-treated AMD solutions.

引用

页数：20

共 95 条

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