Metabotropic glutamate receptor mGluR5 is not involved in the early hemodynamic response

被引：36

作者：

Calcinaghi, Novella ^{[1
]}

Jolivet, Renaud ^{[1
]}

Wyss, Matthias T. ^{[1
,2
]}

Ametamey, Simon M. ^{[3
]}

Gasparini, Fabrizio ^{[4
]}

Buck, Alfred ^{[2
]}

Weber, Bruno ^{[1
]}

机构：

[1] Univ Zurich, Inst Pharmacol & Toxicol, CH-8091 Zurich, Switzerland

[2] Univ Zurich Hosp, Div Nucl Med, PET Ctr, CH-8091 Zurich, Switzerland

[3] ETH, Dept Chem & Appl Biosci, Ctr Radiopharmaceut Sci ETH PSI & USZ, Zurich, Switzerland

[4] Novartis Inst Biomed Res, Basel, Switzerland

来源：

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM | 2011年 / 31卷 / 09期

关键词：

astrocytes; glutamate; G-protein-coupled receptor; metabotropic glutamate receptor mGluR5; neurovascular coupling;

D O I：

10.1038/jcbfm.2011.96

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Activation of astrocytic metabotropic glutamate receptor 5 (mGluR5) is postulated to elicit calcium transients, triggering a chain of events that ultimately regulates cerebral blood flow by changing the tone of smooth muscle cells of nearby arterioles. Using concurrent in vivo optical imaging and determination of receptor occupancy with C-11-ABP688, we report here that blocking similar to 80% of mGluR5 in vivo does not affect transient hemodynamic responses on brief whisker stimulations while transiently reducing neuronal activity as measured by voltage-sensitive dye imaging. Our results show that mechanisms other than activation of mGluR5 are required to trigger the initial hemodynamic response in normal physiological conditions. Journal of Cerebral Blood Flow & Metabolism (2011) 31, 1967; doi:10.1038/jcbfm.2011.96

引用

页码：1967 / 1967

页数：1

共 38 条

[1]

Aronica E., Van Vliet E.A., Mayboroda O.A., Troost D., Lopes Da Silva F.H., Gorter J.A., Upregulation of metabotropic glutamate receptor subtype mGluR3 and mGluR5 in reactive astrocytes in a rat model of mesial temporal lobe epilepsy, European Journal of Neuroscience, 12, 7, pp. 2333-2344, (2000)

[2]

Attwell D., Buchan A.M., Charpak S., Lauritzen M., MacVicar B.A., Newman E.A., Glial and neuronal control of brain blood flow, Nature, 468, pp. 232-243, (2010)

[3]

Ballester-Rosado C.J., Albright M.J., Wu C.S., Liao C.C., Zhu J., Xu J., Lee L.J., Lu H.C., MGluR5 in cortical excitatory neurons exerts both cell-autonomous and-nonautono-mous influences on cortical somatosensory circuit formation, J Neurosci, 30, pp. 16896-909, (2010)

[4]

Berwick J., Johnston D., Jones M., Martindale J., Martin C., Kennerley A.J., Redgrave P., Mayhew J.E.W., Fine detail of neurovascular coupling revealed by spatiotemporal analysis of the hemodynamic response to single whisker stimulation in rat barrel cortex, Journal of Neurophysiology, 99, 2, pp. 787-798, (2008)

[5]

Biber K., Laurie D.J., Berthele A., Sommer B., Tolle T.R., Gebicke-Harter P.-J., Van Calker D., Boddeke H.W.G.M., Expression and signaling of group I metabotropic glutamate receptors in astrocytes and microglia, Journal of Neurochemistry, 72, 4, pp. 1671-1680, (1999)

[6]

Bonvento G., Charbonne R., Correze J.-L., Borredon J., Seylaz J., Lacombe P., Is α-chloralose plus halothane induction a suitable anesthetic regimen for cerebrovascular research?, Brain Research, 665, 2, pp. 213-221, (1994)

[7]

Bushong E.A., Martone M.E., Jones Y.Z., Ellisman M.H., Protoplasmic astrocytes in CA1 stratum radiatum occupy separate anatomical domains, Journal of Neuroscience, 22, 1, pp. 183-192, (2002)

[8]

Cauli B., Hamel E., Revisiting the role of neurons in neurovascular coupling, Front Neuroenergetics, 2, (2010)

[9]

Chaigneau E., Tiret P., Lecoq J., Ducros M., Knopfel T., Charpak S., The relationship between blood flow and neuronal activity in the rodent olfactory bulb, Journal of Neuroscience, 27, 24, pp. 6452-6460, (2007)

[10]

Charpak S., Gahwiler B.H., Glutamate mediates a slow synaptic response in hippocampal slice cultures, Proc Biol Sci, 243, pp. 221-226, (1991)

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