In Situ Crosslinking of Highly Porous Chitosan Scaffolds for Bone Regeneration: Production Parameters and In Vitro Characterization

被引：9

作者：

Kruppke, Benjamin ^{[1
,2
]}

Farack, Jana ^{[1
,2
]}

Sommer, Freya ^{[1
,2
]}

Weil, Simy ^{[3
,4
]}

Aflalo, Eliahu David ^{[3
,4
]}

Wiesmann, Hans-Peter ^{[1
,2
]}

Sagi, Amir ^{[3
,4
]}

Hanke, Thomas ^{[1
,2
]}

机构：

[1] Tech Univ Dresden, Max Bergmann Ctr Biomat, Budapester Str 27, D-01069 Dresden, Germany

[2] Tech Univ Dresden, Inst Mat Sci, Budapester Str 27, D-01069 Dresden, Germany

[3] Ben Gurion Univ Negev, Dept Life Sci, POB 653, IL-84105 Beer Sheva, Israel

[4] Ben Gurion Univ Negev, Natl Inst Biotechnol Negev, POB 653, IL-84105 Beer Sheva, Israel

来源：

MACROMOLECULAR MATERIALS AND ENGINEERING | 2017年 / 302卷 / 10期

关键词：

bioactivity; bone substitute material; chitosan; compressive strength; crosslinking; scaffold; BIOMEDICAL APPLICATIONS; EXTRACELLULAR CALCIUM; COMPOSITE SCAFFOLDS; STROMAL CELLS; TISSUE; BIOACTIVITY; GLUTARALDEHYDE; CYTOTOXICITY; NANOFIBERS; MEMBRANES;

D O I：

10.1002/mame.201700147

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Various methods of chitosan scaffold production are reported in the literature so far. Here, in situ crosslinking with glutaraldehyde is reported for the first time. It combines pore formation and chitosan crosslinking in a single step. This combination allows incorporation of fragile molecules into 3D porous chitosan scaffolds produced by simple and gentle lyophilization. In this study, parameters of in situ crosslinking of porous chitosan scaffold formation as well as their effect on degradation and bioactivity of the scaffolds are examined. The scaffolds are characterized in the context of their prospective application as bone substitute material. The addition of calcium phosphate phases (hydroxyapatite, brushite) to the macroporous chitosan scaffolds allows manipulation of the bioactivity that is investigated by incubation in simulated body fluid (SBF). The bioactivity is significantly influenced by the modus of changing the fluid (static, daily-, and twice-a-week change). Scaffolds are morphologically characterized by means of scanning electron microscopy, and the mechanical stability is tested after incubation in SBF and phosphate-buffered saline.

引用

页数：13

共 45 条

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