High-performance liquid chromatography separation of the (S,S)- and (R,S)-forms of S-adenosyl-L-methionine

被引：14
|
作者：
Zhang, Jianyu [1 ,2 ]
Klinman, Judith P. [1 ,2 ,3 ]
机构：
[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Calif Inst Quantitat Biosci, Berkeley, CA 94720 USA
[3] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
来源：
ANALYTICAL BIOCHEMISTRY | 2015年 / 476卷
基金：
美国国家卫生研究院;
关键词：
S-adenosyl-L-methionine; Chiral resolution; Diastereoisomer; High-performance liquid chromatography; Radioactive; Separation; ADENOSYLMETHIONINE; METHYLTRANSFERASES; INSTABILITY; SYNTHASE; SULFUR;
D O I：
10.1016/j.ab.2015.02.004
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
S-adenosyl-i-methionine (AdoMet), an important biological cofactor, exists in two chiral forms, (S,S)- and (R,S)-, only the former of which is biologically active. Here, we have developed a chromatographic method to obtain pure (S,S)-AdoMet using a single C18 column. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码：81 / 83
页数：3
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