Isolation and characterization of microsatellite markers from Musa balbisiana

被引:32
作者
Buhariwalla, HK [1 ]
Jarret, RL
Jayashree, B
Crouch, JH
Ortiz, R
机构
[1] Int Crops Res Inst Semi Arid Trop, MS Swaminathan Genom Lab, Patancheru 502324, Andhra Pradesh, India
[2] ARS, Plant Genet Resources Unit, USDA, Griffin, GA 30223 USA
[3] CIMMYT, Mexico City 06600, DF, Mexico
来源
MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 02期
关键词
B genome; microsatellite; Musa; polymorphism; polyploid;
D O I
10.1111/j.1471-8286.2005.00916.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This is the first report of targeted development of B genome microsatellite markers in Musa. A total of 44 sequences with microsatellites were isolated from an enriched library of Musa balbisiana cv. 'Tani' (BB genome). Of these, 25 were polymorphic when screened on 14 diverse diploid and triploid Musa accessions. The number of alleles detected by each marker ranged between one and seven. All 25 microsatellite markers generated amplification products in all species and genome complements. These new microsatellite markers fill an important gap for diversity assessment and linkage mapping studies in plantain (AAB) and cooking banana (ABB).
引用
收藏
页码:327 / 330
页数:4
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