Fisetin Inhibits Lipopolysaccharide-Induced Macrophage Activation and Dendritic Cell Maturation

被引：47

作者：

Liu, Sheng-Hung ^{[1
,2
,3
]}

Lin, Chao-Hsiung ^{[4
,5
]}

Hung, Shih-Kai ^{[1
,6
]}

Chou, Jen-Hwey ^{[1
]}

Chi, Chin-Wen ^{[2
,7
]}

Fu, Shu-Ling ^{[1
,3
]}

机构：

[1] Natl Yang Ming Univ, Inst Tradit Med, Taipei 11221, Taiwan

[2] Natl Yang Ming Univ, Dept & Inst Pharmacol, Taipei 11221, Taiwan

[3] Natl Yang Ming Univ, Program Mol Med, Taipei 11221, Taiwan

[4] Natl Yang Ming Univ, Dept Life Sci, Taipei 11221, Taiwan

[5] Natl Yang Ming Univ, Inst Genome Sci, Taipei 11221, Taiwan

[6] Buddhist Dalin Tzu Chi Gen Hosp, Dept Radiat Oncol, Chiayi 62247, Taiwan

[7] Taipei Vet Gen Hosp, Dept Med Res & Educ, Taipei 11217, Taiwan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2010年 / 58卷 / 20期

关键词：

Fisetin; dendritic cell; macrophage; NF-kappa B; anti-inflammatory; NF-KAPPA-B; ADAPTIVE IMMUNE-RESPONSES; IMMUNOSTIMULATORY FUNCTION; EPITHELIAL-CELLS; ANTIINFLAMMATORY ACTIVITY; TLR4-SIGNALING PATHWAYS; OXIDATIVE-STRESS; RHUS-VERNICIFLUA; HUMAN BASOPHILS; BONE-MARROW;

D O I：

10.1021/jf1017093

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Macrophages and dendritic cells are required for initiating innate immunity and adaptive immunity. Aberrant activation of macrophages and dendritic cells can cause detrimental immune responses; thus, agents effectively modulating their functions are of great clinical value. We herein investigated whether fisetin, a flavonoid prevalently present in fruits and vegetables, could inhibit macrophage activation and dendritic cell maturation. Fisetin suppressed LPS-induced NF-kappa B activation, expression of pro-inflammatory proteins (TNF-alpha and iNOS), MMP-9 activity, and phagocytic activity in macrophages. Furthermore, upon LPS-induced dendritic cell maturation, fisetin at nontoxic concentrations suppressed the expression of costimulatory molecules (CD80 and CD86), the production of cytokines (IL-12, IL-6, and TNF-alpha), and the endocytic activity of dendritic cells. Fisetin treatment significantly attenuated migration of dendritic cells into spleens and dendritic cell-mediated T cell activation in LPS-treated mice. Collectively, our data reveal that fisetin inhibits macrophage activation and impairs functional maturation of dendritic cells.

引用

页码：10831 / 10839

页数：9

共 45 条

[1] Epigallocatechin-3-gallate, constituent of green tea, suppresses the LPS-induced phenotypic and functional maturation of murine dendritic cells through inhibition of mitogen-activated protein kinases and NF-κB [J].