Tumor necrosis factor-α internalization and degradation by isolated hepatocytes of rats exposed to ethanol

被引:4
作者
Deaciuc, IV
Alappat, JM
D'Souza, NB
Van Thiel, DH
McClain, CJ
机构
[1] Univ Kentucky, Coll Med, Dept Med, GI, Lexington, KY 40536 USA
[2] Louisiana State Univ, Med Ctr, Dept Physiol, New Orleans, LA 70112 USA
[3] Louisiana State Univ, Med Ctr, Dept Med Pulm Crit Care, New Orleans, LA 70112 USA
关键词
cytokines; postbinding events; intracellular degradation; alcohol;
D O I
10.1016/S0741-8329(97)00174-2
中图分类号
R194 [卫生标准、卫生检查、医药管理];
学科分类号
摘要
Internalization and degradation of human recombinant [I-125]TNF-alpha was studied in hepatocytes isolated from rats exposed to ethanol (EtOH) either acutely (IP injection, 2.2 g kg(-1) b.wt.) or chronically (14-16 weeks of EtOH feeding in liquid diet). Both acute and chronic EtOH exposure diminished cytokine binding to the cell-surface receptors. In the acute group, EtOH increased internalization of the cytokine, accelerated its disapperance from the cell surface, and markedly reduced its conversion into acid-soluble I-125-containing compounds. In the chronic group, EtOH did not markedly affect these parameters. Internalization and degradation of the cytokine in the chronic group was much lower than in the acute group. It is concluded that EtOH interferes not only with the cytokine binding to the cell-surface receptors, as demonstrated in previous studies, but also with postbinding events, such as internalization and intracellular degradation of TNF-alpha. Possible mechanisms of action of EtOH are discussed. (C) 1998 Elsevier Science Inc.
引用
收藏
页码:125 / 133
页数:9
相关论文
共 37 条
[1]  
AGGARWAL BB, 1986, J BIOL CHEM, V261, P3652
[2]  
AGGARWAL BB, 1987, J BIOL CHEM, V262, P16450
[3]  
BAJZER Z, 1989, J BIOL CHEM, V264, P13623
[4]  
BERMUDEZ LE, 1991, LYMPHOKINE CYTOK RES, V10, P413
[5]  
CHAMPSI J, 1995, IMMUNOLOGY, V84, P549
[6]   CHRONIC ETHANOL ADMINISTRATION IMPAIRS RECEPTOR-MEDIATED ENDOCYTOSIS OF EPIDERMAL GROWTH-FACTOR BY RAT HEPATOCYTES [J].
DALKE, DD ;
SORRELL, MF ;
CASEY, CA ;
TUMA, DJ .
HEPATOLOGY, 1990, 12 (05) :1085-1091
[7]   EFFECT OF ACUTE AND CHRONIC ALCOHOL ADMINISTRATION TO RATS ON THE EXPRESSION OF INTERLEUKIN-6 CELL-SURFACE RECEPTORS OF HEPATIC PARENCHYMAL AND NONPARENCHYMAL CELLS [J].
DEACIUC, IV ;
ALAPPAT, JM ;
DSOUZA, NB .
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 1994, 18 (05) :1207-1214
[8]   TUMOR-NECROSIS-FACTOR-ALPHA CELL-SURFACE RECEPTORS OF LIVER PARENCHYMAL AND NONPARENCHYMAL CELLS DURING ACUTE AND CHRONIC ALCOHOL ADMINISTRATION TO RATS [J].
DEACIUC, IV ;
DSOUZA, NB ;
SPITZER, JJ .
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 1995, 19 (02) :332-338
[9]   Interleukin-6 and tumor necrosis factor-alpha clearance and metabolism in vivo and by the isolated, perfused liver in the rat: Effect of acute alcohol administration [J].
Deaciuc, IV ;
Alappat, JM ;
McDonough, KH ;
DSouza, NB .
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 1996, 20 (02) :293-301
[10]  
DEVIERE J, 1989, CLIN EXP IMMUNOL, V77, P221