Studies on the antioxidant potential of flavones of Allium vineale isolated from its water-soluble fraction

被引：110

作者：

Demirtas, Ibrahim ^{[1
]}

Erenler, Ramazan ^{[2
]}

Elmastas, Mahfuz ^{[2
]}

Goktasoglu, Ahmet ^{[2
]}

机构：

[1] Cankiri Karatekin Univ, Dept Chem, Fac Sci, TR-18100 Cankiri, Turkey

[2] Gaziosmanpasa Univ, Fac Sci & Art, Dept Chem, Plant Res Lab, TR-60240 Tokat, Turkey

来源：

FOOD CHEMISTRY | 2013年 / 136卷 / 01期

关键词：

Allium vineale; Water-soluble flavonoid extraction; Antioxidant activity; Flavonoids; ANTIMICROBIAL ACTIVITIES; GENUS ALLIUM; EXTRACTS; L; FRUIT; DISEASE; ONIONS; OXYGEN;

D O I：

10.1016/j.foodchem.2012.07.086

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The aim of this work was to examine the chemical constituents and antioxidant potential of water-soluble fractions from the commonly consumed vegetable, Allium vineale. The water-soluble fraction, containing phenolic compounds, was extracted with ethyl acetate to obtain flavonoids which were separated and purified by repeated column chromatography over Sephadex LH-20, RP C18 and silica gel. The isolated compounds were identified according to their physicochemical properties and spectral data (UV, HPLC-TOF/MS, H-1 NMR, C-13 NMR and 2D NMR). Three flavonoids were isolated and identified as chrysoeriol-7-O-[2 ''-O-E-feruloyl]-beta-D-glucoside (1), chrysoeriol (2), and isorhamnetin-3-beta-D-glucoside (3). Antioxidant studies of the aqueous extract and three isolated compounds, 1, 2, 3, were undertaken and they were found to have significant antioxidant activity. Antioxidant activities were evaluated for total antioxidant activity by the ferric thiocyanate method, ferric ion (Fe3+) reducing antioxidant power assay (FRAP), ferrous ion (Fe2+) metal chelating activity, and DPPH free radical-scavenging activity. The water-soluble ethyl acetate and methanol extraction methods were also compared using HPLC-TOF/MS. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：34 / 40

页数：7