Molecular cloning and expression profile of an abiotic stress and hormone responsive MYB transcription factor gene from Panax ginseng

被引：25

作者：

Afrin, Sadia ^{[1
]}

Zhu, Jie ^{[1
]}

Cao, Hongzhe ^{[1
]}

Huang, Jingjia ^{[1
]}

Xiu, Hao ^{[1
]}

Luo, Tiao ^{[1
]}

Luo, Zhiyong ^{[1
]}

机构：

[1] Cent South Univ, Sch Life Sci, State Key Lab Med Genet, Mol Biol Res Ctr, Changsha 410078, Hunan, Peoples R China

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2015年 / 47卷 / 04期

基金：

中国国家自然科学基金;

关键词：

abiotic stress; gene expression; hormone; MYB transcription factor; Panax ginseng; DROUGHT TOLERANCE; OVER-EXPRESSION; COLD; SALT; OVEREXPRESSION; PROTEIN; ACID; IDENTIFICATION; EVOLUTIONARY; OSMYB3R-2;

D O I：

10.1093/abbs/gmv012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The v-myb avian myeloblastosis viral oncogene homolog (MYB) family constitutes one of the most abundant groups of transcription factors and plays vital roles in developmental processes and defense responses in plants. A ginseng (Panax ginseng C.A. Meyer) MYB gene was cloned and designated as PgMYB1. The cDNA of PgMYB1 is 762 base pairs long and encodes the R2R3-type protein consisting 238 amino acids. Subcellular localization showed that PgMYB1-mGFP5 fusion protein was specifically localized in the nucleus. To understand the functional roles of PgMYB1, we investigated the expression patterns of PgMYB1 in different tissues and under various conditions. Quantitative real-time polymerase chain reaction and western blot analysis showed that PgMYB1 was expressed at higher level in roots, leaves, and lateral roots than in stems and seeds. The expression of PgMYB1 was up-regulated by abscisic acid, salicylic acid, NaCl, and cold (chilling), and down-regulated by methyl jasmonate. These results suggest that PgMYB1 might be involved in responding to environmental stresses and hormones.

引用

页码：267 / 277

页数：11

共 52 条

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