DW-F5: A novel formulation against malignant melanoma from Wrightia tinctoria

被引：20

作者：

Antony, Jayesh ^{[1
]}

Saikia, Minakshi ^{[1
]}

Vinod, V. ^{[1
]}

Nath, Lekshmi R. ^{[1
]}

Katiki, Mohana Rao ^{[2
]}

Murty, M. S. R. ^{[2
]}

Paul, Anju ^{[1
]}

Shabna, A. ^{[1
]}

Chandran, Harsha ^{[1
]}

Joseph, Sophia Margaret ^{[1
]}

Kumar, Nishanth S. ^{[3
]}

Panakkal, Elizabeth Jayex ^{[1
]}

Sriramya, I., V ^{[1
]}

Sridivya, I., V ^{[1
]}

Ran, Sophia ^{[4
]}

Sankar, S. ^{[5
]}

Rajan, Easwary ^{[6
]}

Anto, Ruby John ^{[1
]}

机构：

[1] Rajiv Gandhi Ctr Biotechnol, Div Canc Res, Thiruvananthapuram 695014, Kerala, India

[2] Indian Inst Chem Technol, Med Chem & Pharmacol Div, Hyderabad 500007, Andhra Pradesh, India

[3] CSIR, NIIST, Agroproc & Nat Prod Div, Thiruvananthapuram 695019, Kerala, India

[4] So Illinois Univ, Sch Med, Dept Med Microbiol Immunol & Cell Biol, Springfield, IL USA

[5] Govt Med Coll, Dept Pathol, Thiruvananthapuram 695011, Kerala, India

[6] Sree Kerala Varma Coll, Dept Chem, Trichur 680011, Kerala, India

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

关键词：

FACTOR-KAPPA-B; SIGNALING PATHWAY; LINEAGE SURVIVAL; MASTER REGULATOR; DOWN-REGULATION; ACTIVATED BRAF; IN-VIVO; TRYPTANTHRIN; PROLIFERATION; INHIBITION;

D O I：

10.1038/srep11107

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Wrightia tinctoria is a constituent of several ayurvedic preparations against skin disorders including psoriasis and herpes, though not yet has been explored for anticancer potential. Herein, for the first time, we report the significant anticancer properties of a semi-purified fraction, DW-F-5, from the dichloromethane extract of W. tinctoria leaves against malignant melanoma. DW-F-5 exhibited anti-melanoma activities, preventing metastasis and angiogenesis in NOD-SCID mice, while being nontoxic in vivo. The major pathways in melanoma signaling mediated through BRAF, WNT/beta-catenin and Akt-NF-kappa B converging in MITF-M, the master regulator of melanomagenesis, were inhibited by DW-F-5, leading to complete abolition of MITF-M. Purification of DW-F-5 led to the isolation of two cytotoxic components, one being tryptanthrin and the other being an unidentified aliphatic fraction. The overall study predicts Wrightia tinctoria as a candidate plant to be further explored for anticancer properties and DW-F-5 as a forthcoming drug formulation to be evaluated as a chemotherapeutic agent against malignant melanoma.

引用

页数：14

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