Proliferation and differentiation of adipose-derived stem cells on naturally derived scaffolds

被引：71

作者：

Flynn, Lauren E. ^{[1
]}

Prestwich, Glenn D. ^{[2
,3
]}

Semple, John L. ^{[4
,5
,6
]}

Woodhouse, Kimberly A. ^{[1
,6
,7
]}

机构：

[1] Univ Toronto, Dept Chem Engn & Appl Chem, Toronto, ON M5S 3E5, Canada

[2] Univ Utah, Ctr Therapeut Biomat, Salt Lake City, UT 84108 USA

[3] Univ Utah, Dept Med Chem, Salt Lake City, UT 84108 USA

[4] Univ Toronto, Div Plast Surg, Dept Surg, Toronto, ON M5G 1L5, Canada

[5] Womens Coll Hosp, Toronto, ON M5S 1B2, Canada

[6] Sunnybrook Hlth Sci Ctr, Adv Regenerat Tissue Engn Ctr, Toronto, ON M4N 3M5, Canada

[7] Queens Univ, Dept Chem Engn, Kingston, ON K7L 3N6, Canada

来源：

BIOMATERIALS | 2008年 / 29卷 / 12期

关键词：

adipose tissue engineering; scaffold; extracellular matrix; hyaluronan; stem cells;

D O I：

10.1016/j.biomaterials.2007.12.028

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A tissue-engineered substitute that facilitates large-volume regeneration of the subcutaneous adipose tissue layer is needed for reconstructive plastic surgery. Towards this goal, we describe the in vitro culture of primary human adipose-derived stem cells (ASC) seeded into placental decellular matrix (PDM) and cross-linked hyaluronan (XLHA) scaffolds. Specifically, we evaluated cellular proliferation and adipogenic differentiation in the PDM, XLHA, and PDM combined with XLHA scaffolds. Cellular proliferation, viability, and glucose consumption were determined prior to the induction of differentiation. Adipogenesis within each of the scaffolds was investigated through gene expression analysis using end point and real time reverse transcriptase polymerase chain reaction (RT-PCR). The results indicate that the cell-adhesive PDM scaffolds facilitated proliferation and viability, while differentiation was augmented when the cells were encapsulated in the non-adhesive XLHA gels. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：1862 / 1871

页数：10

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