Microbial Diversity Analysis of Reservoirs after Polymer Flooding in Daqing Oil Field at the Earlier Stage of Microbial Profile Modification

被引：3

作者：

Huang, Yonghong ^{[1
]}

Ren, Guoling ^{[1
]}

Yuan, Hongmei ^{[1
]}

Wei, Li ^{[2
]}

Wu, Xiaolin ^{[3
]}

Song, Kaoping ^{[4
]}

机构：

[1] Daqing Normal Univ, Coll Life Sci, Daqing 163712, Peoples R China

[2] Harbin Inst Technol, Coll Environm Engn, Harbin 50001, Peoples R China

[3] Daqing Oil Field Co Ltd, Explorat & Dev Res Inst, Daqing 163712, Peoples R China

[4] Daqing Petrol Univ, Coll Petrol Engn, Daqing 163318, Peoples R China

来源：

ENVIRONMENT MATERIALS AND ENVIRONMENT MANAGEMENT PTS 1-3 | 2010年 / 113-116卷

关键词：

microbial profile modification; oil reservoirs after polymer flooding; 16S rDNA; gene clone library; HIGH-TEMPERATURE;

D O I：

10.4028/www.scientific.net/AMR.113-116.835

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

To gain a better understanding of the mechanism and technology of microbial enhanced oil recovery, microbial community structure and diversity of reservoirs after polymer flooding in Daqing oil field at the Earlier Stage of microbial profile modification were studied. 16S rDNA gene clone library was used to assess the structure and diversity of microbial community. The results showed that the dominant microbes of the earlier stage of microbial profile modification are uncultured bacterium, comprising 88.6% of library clones. The cultured strains are composed of Epsilonproteobacteria(5.7%), Gammaproteobacteria(4.7%) and Firmicutes (1%). Among the Epsilonproteobacteria, Sulfuricurvum accounts for 4.7% of the cultured strains of library clones and Arcobacter accounts for 1%. Besides, the dominant communities also include Pseudomonas and Moorella.

引用

页码：835 / +

页数：2

共 12 条

[1] ARCHAEA IN COASTAL MARINE ENVIRONMENTS [J].

DELONG, EF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (12) :5685-5689

[2] Microbial diversity in production waters of a low-temperature biodegraded oil reservoir [J].

Grabowski, A ;

Nercessian, O ;

Fayolle, F ;

Blanchet, D ;

Jeanthon, C .

FEMS MICROBIOLOGY ECOLOGY, 2005, 54 (03) :427-443

[3]

Huang YH, 2009, J DAQING PETROLEUM I, V33, P63

[4] Phylogenetic diversity of the archaeal community in a continental high-temperature, water-flooded petroleum reservoir [J].

Li, Hui ;

Yang, Shi-Zhong ;

Mu, Bo-Zhong .

CURRENT MICROBIOLOGY, 2007, 55 (05) :382-388

[5]

LUO Q, 2005, GEOLOGICAL SCI TECHN, V25, P101

[6] Culture-dependent and culture-independent characterization of microbial assemblages associated with high-temperature petroleum reservoirs [J].

Orphan, VJ ;

Taylor, LT ;

Hafenbradl, D ;

Delong, EF .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (02) :700-711

[7]

[佘跃惠 She Yuehui], 2005, [生态学报, Acta Ecologica Sinica], V25, P237

[8] Characterization of 16S rRNA genes from oil field microbial communities indicates the presence of a variety of sulfate-reducing, fermentative, and sulfide-oxidizing bacteria [J].

Voordouw, G ;

Armstrong, SM ;

Reimer, MF ;

Fouts, B ;

Telang, AJ ;

Shen, Y ;

Gevertz, D .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (05) :1623-1629

[9]

WANG J, 2007, CHEM BIOENGINEERING, V24, P12

[10] Molecular characterization of bacterial populations in petroleum-contaminated groundwater discharged from underground crude oil storage cavities [J].

Watanabe, K ;

Kodama, Y ;

Syutsubo, K ;

Harayama, S .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (11) :4803-+

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