Nucleotide sequence analysis and expression of the alginate lyase gene from Pseudomonas sp. W7 in Escherichia coli

被引：0

作者：

Lee, JH

Kang, JH

Kim, YO

Kim, JM

Kong, IS ^{[1
]}

机构：

[1] Pukyong Natl Univ, Dept Biotechnol & Bioengn, Pusan 608737, South Korea

[2] Yosu Natl Univ, Dept Biotechnol, Yosu 550749, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 1998年 / 8卷 / 05期

关键词：

Pseudomonas; alginate lyase; nucleotide sequence;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The gene encoding alginate lyase was isolated from a library constructed with the vector, pUC19, and expressed in Escherichia coli. The nucleotide sequence of the cloned alginate lyase gene (ALY) from Pseudomonas sp. W7 was determined. The nucleotide sequence revealed a 1, 035 bp open reading frame (ORF), encoding 345 amino acid residues with a calculated molecular mass of 37,478 Da. The N-terminal amino acid sequences (15 residues) of purified alginate lyase corresponded to that of the deduced amino acid sequence.

引用

页码：531 / 535

页数：5

共 15 条

[1] Cloning, sequencing and overexpression in Escherichia coli of the alginate-lyase-encoding aly gene of Pseudomonas alginovora: Identification of three classes of alginate lyases [J].