UV-C radiation induces apoptotic-like changes in Arabidopsis thaliana

被引：135

作者：

Danon, A ^{[1
]}

Gallois, P ^{[1
]}

机构：

[1] Univ Perpignan, Lab Physiol & Biol Mol Plantes, UMR CNRS 5545, F-66860 Perpignan, France

来源：

FEBS LETTERS | 1998年 / 437卷 / 1-2期

关键词：

programmed cell death; ultraviolet-C light; plant; terminal deoxynucleotidyl transferase-mediated dUTP; nick end labelling; protoplast; DNA ladder;

D O I：

10.1016/S0014-5793(98)01208-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

With a view to studying programmed cell death in plants at the molecular level, we report here for the first time that apoptotic-like changes are induced by UV radiation in plant nuclei. In Arabidopsis thaliana seedlings a UV-C dose of 10-50 kJ/m(2) induces an oligonucleosomal DNA fragmentation which is reminiscent of the apoptotic DNA ladder described in animal cells, This DNA fragmentation was also detected in situ in protoplast nuclei as soon as 2 h after UV-C treatment. Moreover, UV-C induced a nuclear morphology characteristic of animal apoptotic nuclei. We propose that UV-C induction constitutes a powerful tool to compare the cellular response to irreversible UV damage in plants to that in animals and to study programmed cell death in A. thaliana, (C) 1998 Federation of European Biochemical Societies,

引用

页码：131 / 136

页数：6

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