Enteropathogenic Escherichia coli strains carrying genes encoding the PER-2 and TEM-116 extended-spectrum β-lactamases isolated from children with diarrhea in Uruguay

被引：53

作者：

Vignoli, R

Varela, G

Mota, MI

Cordeiro, NF

Power, P

Ingold, E

Gadea, P

Sirok, A

Schelotto, F

Ayala, JA

Gutkind, G ^{[1
]}

机构：

[1] Univ Buenos Aires, Fac Farm & Bioquim, Catedra Microbiol, RA-1113 Buenos Aires, DF, Argentina

[2] Univ Republica, Fac Med, Inst Higiene, Dept Bacteriol & Virol, Montevideo 11600, Uruguay

[3] Univ Autonoma Madrid, CSIC, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 06期

关键词：

D O I：

10.1128/JCM.43.6.2940-2943.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We studied 13 extended-spectrum P-lactamase (ESBL)-producing enteropathogenic Escherichia coli isolates from children suffering acute diarrhea in Uruguay. ESBL characterization in crude extracts showed a single band at pI 5.4. PCR amplification and sequencing data allowed identification of bla(PER-2) and bla(TEM-116). Retrospective analysis suggests that these strains were disseminated in the community, even if unnoticed, prior to their access to the hospital environment more than a decade ago.

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页码：2940 / 2943

页数：4

相关论文

共 23 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2] Characterization of beta-lactamase gene bla(PER-2,) which encodes an extended-spectrum class A beta-lactamase [J].

Bauernfeind, A ;

Stemplinger, I ;

Jungwirth, R ;

Mangold, P ;

Amann, S ;

Akalin, E ;

Ang, O ;

Bal, C ;

Casellas, JM .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (03) :616-620

[3]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[4] Growing group of extended-spectrum β-lactamases:: The CTX-M enzymes [J].

Bonnet, R .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (01) :1-14

[5] Extended-spectrum β-lactamases in the 21st century:: Characterization, epidemiology, and detection of this important resistance threat [J].

Bradford, PA .

CLINICAL MICROBIOLOGY REVIEWS, 2001, 14 (04) :933-951

[6] Characterization of a chromosomally encoded extended-spectrum class A β-lactamase from Kluyvera cryocrescens [J].

Decousser, JW ;

Poirel, L ;

Nordmann, P .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (12) :3595-3598

[7] DETECTION AND CHARACTERIZATION OF THE EAE GENE OF SHIGA-LIKE TOXIN-PRODUCING ESCHERICHIA-COLI USING POLYMERASE CHAIN-REACTION [J].

GANNON, VPJ ;

RASHED, M ;

KING, RK ;

THOMAS, EJG .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (05) :1268-1274

[8] IDENTIFICATION OF ENTEROPATHOGENIC ESCHERICHIA-COLI BY PCR-BASED DETECTION OF THE BUNDLE-FORMING PILUS GENE [J].

GUNZBURG, ST ;

TORNIEPORTH, NG ;

RILEY, LW .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (05) :1375-1377

[9] β-lactamases of Kluyvera ascorbata, probable progenitors of some plasmid-encoded CTX-M types [J].

Humeniuk, C ;

Arlet, G ;

Gautier, V ;

Grimont, P ;

Labia, R ;

Philippon, A .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (09) :3045-3049

[10] Molecular characterization of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Eschetichia coli from a Korean nationwide survey [J].

Jeong, SH ;

Bae, IK ;

Lee, JH ;

Sohn, SG ;

Kang, GH ;

Jeon, GJ ;

Kim, YH ;

Jeong, BC ;

Lee, SH .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (07) :2902-2906