Cytoplasmic hybrid (cybrid) cell lines as a practical model for mitochondriopathies

被引：111

作者：

Wilkins, Heather M. ^{[1
,2
]}

Carl, Steven M. ^{[1
,2
]}

Swerdlow, Russell H. ^{[1
,2
,3
,4
]}

机构：

[1] Univ Kansas, Med Ctr, Dept Neurol, Kansas City, KS 66160 USA

[2] Univ Kansas, Med Ctr, Alzheimers Dis Ctr, Kansas City, KS 66160 USA

[3] Univ Kansas, Med Ctr, Dept Mol & Integrat Physiol, Kansas City, KS 66160 USA

[4] Univ Kansas, Med Ctr, Dept Biochem & Mol Biol, Kansas City, KS 66160 USA

来源：

REDOX BIOLOGY | 2014年 / 2卷

关键词：

Cybrid; Mitochondria; Mitochondrial DNA; Rho zero; HEREDITARY OPTIC NEUROPATHY; OXYGEN SPECIES PRODUCTION; DISEASE NEURONAL CYBRIDS; MTDNA CONTROL-REGION; COMPLEX I DEFECT; ALZHEIMERS-DISEASE; PARKINSONS-DISEASE; POINT MUTATION; DNA MUTATIONS; CHLORAMPHENICOL RESISTANCE;

D O I：

10.1016/j.redox.2014.03.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytoplasmic hybrid (cybrid) cell lines can incorporate human subject mitochondria and perpetuate its mitochondrial DNA (mtDNA)-encoded components. Since the nuclear background of different cybrid lines can be kept constant, this technique allows investigators to study the influence of mtDNA on cell function. Prior use of cybrids has elucidated the contribution of mtDNA to a variety of biochemical parameters, including electron transport chain activities, bioenergetic fluxes, and free radical production. While the interpretation of data generated from cybrid cell lines has technical limitations, cybrids have contributed valuable insight into the relationship between mtDNA and phenotype alterations. This review discusses the creation of the cybrid technique and subsequent data obtained from cybrid applications. (C) 2014 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license

引用

页码：619 / 631

页数：13

共 167 条

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