Crocins transport in Crocus sativus: The long road from a senescent stigma to a newborn corm

被引:41
作者
Rubio-Moraga, Angela [1 ]
Trapero, Almudena [1 ]
Ahrazem, Oussama [1 ]
Gomez-Gomez, Lourdes [1 ]
机构
[1] Univ Castilla La Mancha, ETSIA, E-02071 Albacete, Spain
关键词
Apocarotenoids; Corm; Flower senescence; Saffron; Stigma; Transport; POLLEN-TUBE GROWTH; GENE-EXPRESSION; LEAF; TEMPERATURE; GERMINATION; DIOXYGENASES; INHIBITION; COMPONENTS; COROLLA; ENZYMES;
D O I
10.1016/j.phytochem.2010.05.026
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Saffron, the desiccated stigmas of Crocus sativus, is highly appreciated by its peculiar colour, flavour and aroma. The main compounds that accumulated throughout stigma development in C. sativus are crocetin, its glucoside derivatives, crocins, and picrocrocin, all of which increased as stigmas reached a fully developed stage. After anthesis, and in the absence of fertilization, the flower enters in a senescence programme, which represents the ultimate stage of floral development and results in wilting of whole flower. The programmed senescence of flowers allows the removal of a metabolically active tissue. We studied the composition of saffron apocarotenoids during the senescence of C sativus flowers, and observed that changes in crocins were due to their transport from the senescent stigma to the ovaries and the developing corm. Afterwards, deglucosylation of crocins in these tissues results in crocetin accumulation. This mobilization mimics the export to storage cells (resorbed) of different compounds during leaf senescence avoiding loss of nutrients in leaves that would otherwise be cycled back into the soil system through leaf litter decomposition. In C sativus, the resorbed apocarotenoids are stored within the developing corm, where they are not further detected in the advanced stages of development, suggesting that they are metabolized during the early and active phases of corm development, where the glucose molecules from crocins might contribute to cell initiation and elongation. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1506 / 1513
页数:8
相关论文
共 56 条
[1]   BETA-GLUCOSIDES OF AROMA COMPONENTS FROM PETALS OF ROSA SPECIES - ASSAY, OCCURRENCE, AND BIOSYNTHETIC IMPLICATIONS [J].
ACKERMANN, IE ;
BANTHORPE, DV ;
FORDHAM, WD ;
KINDER, JP ;
POOTS, I .
JOURNAL OF PLANT PHYSIOLOGY, 1989, 134 (05) :567-572
[2]   Differential expression of putative 9-cis-epoxycarotenoid dioxygenases and abscisic acid accumulation in water stressed vegetative and reproductive tissues of citrus [J].
Agusti, Javier ;
Zapater, Marta ;
Iglesias, Domingo J. ;
Cercos, Manuel ;
Tadeo, Francisco R. ;
Talon, Manuel .
PLANT SCIENCE, 2007, 172 (01) :85-94
[3]   Chemical identification and functional analysis of apocarotenoids involved in the development of arbuscular mycorrhizal symbiosis [J].
Akiyama, Kohki .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2007, 71 (06) :1405-1414
[4]   Plant carotenoid cleavage oxygenases and their apocarotenoid products [J].
Auldridge, Michele E. ;
McCarty, Donald R. ;
Klee, Harry J. .
CURRENT OPINION IN PLANT BIOLOGY, 2006, 9 (03) :315-321
[5]   PHYSIOLOGICAL-CHANGES ACCOMPANYING SENESCENCE IN THE EPHEMERAL DAYLILY FLOWER [J].
BIELESKI, RL ;
REID, MS .
PLANT PHYSIOLOGY, 1992, 98 (03) :1042-1049
[6]   ONSET OF PHLOEM EXPORT FROM SENESCENT PETALS OF DAYLILY [J].
BIELESKI, RL .
PLANT PHYSIOLOGY, 1995, 109 (02) :557-565
[7]  
Bolandi M., 2006, V43, P323
[8]  
Britton G., 1998, CAROTENOIDS, P13
[9]  
BUDDHIKA AM, 2007, J NATL SCI FOUND SRI, V35, P259
[10]   Oxidative remodeling of plastid carotenoids [J].
Camara, B ;
Bouvier, F .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2004, 430 (01) :16-21