Identification of two conserved cis-acting elements, MYCS and P1BS, involved in the regulation of mycorrhiza-activated phosphate transporters in eudicot species

被引:112
作者
Chen, Aiqun [1 ]
Gu, Mian [1 ]
Sun, Shubin [1 ]
Zhu, Lingling [1 ]
Hong, Shuai [1 ]
Xu, Guohua [1 ]
机构
[1] Nanjing Agr Univ, Coll Resources & Environm Sci, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China
基金
中国国家自然科学基金;
关键词
cis-elements; mycorrhiza; phosphate transporters; promoter; solanaceous species; ARBUSCULE-CONTAINING CELLS; LEGHEMOGLOBIN GENE VFLB29; TRANSCRIPTION FACTOR; FUNCTIONAL-ANALYSIS; ROOT DEVELOPMENT; INFECTED-CELLS; STARVATION; EXPRESSION; GROWTH; PLANT;
D O I
10.1111/j.1469-8137.2010.03556.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In this study, six putative promoter regions of phosphate transporter Pht1; 3, Pht1; 4 and Pht1; 5 genes were isolated from eggplant and tobacco using the inverse polymerase chain reaction (iPCR). The isolated sequences show evolutionary conservation and divergence within/between the two groups of Pht1; 3 and Pht1; 4/Pht1; 5. Histochemical analyses showed that all six promoter fragments were sufficient to drive beta-glucuronidase (GUS) expression specifically in arbuscular mycorrhizal (AM) tobacco roots and were confined to distinct cells containing AM fungal structures (arbuscules or intracellular hyphae). A series of promoter truncation and mutation analyses combined with phylogenetic footprinting of these promoters revealed that at least two cis-regulatory elements - the mycorrhiza transcription factor binding sequence (MYCS) first identified in this study and P1BS - mediated the transcriptional activation of the AM-mediated inorganic phosphate (Pi) transporter genes. Deletion or partial mutation of either of the two motifs in the promoters could cause a remarkable decrease, or even complete absence, of the promoter activity. Our results propose that uptake of inorganic phosphate (Pi) by AM fungi is regulated, at least partially, in an MYCS- and P1BS-dependent manner in eudicot species. Our finding offers new insights into the molecular mechanisms underlying the coordination between the AM and the Pi signalling pathways.
引用
收藏
页码:1157 / 1169
页数:13
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