Signaling Pathway Involved in the Immunomodulatory Effect of Ganoderma atrum Polysaccharide in Spleen Lymphocytes

被引：61
作者：
Yu, Qiang [1 ]
Nie, Shao-Ping [1 ]
Wang, Jun-Qiao [1 ]
Huang, Dan-Fei [1 ]
Li, Wen-Juan [1 ]
Xie, Ming-Yong [1 ]
机构：
[1] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2015年 / 63卷 / 10期
基金：
中国国家自然科学基金;
关键词：
Ganoderma atrum polysaccharide; spleen lymphocytes; immunomodulatory; effect; signaling pathway; interleukin-2; EVOKES ANTITUMOR-ACTIVITY; ACTIVATED PROTEIN-KINASE; FACTOR-ALPHA SECRETION; KAPPA-B; CALCINEURIN; CALCIUM; NFAT; TRANSCRIPTION; ARCHITECTURE; BINDING;
D O I：
10.1021/acs.jafc.5b00028
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
The aim of this study was to investigate the molecular mechanism underlying the immunomodulatory effect of Ganoderma atrum polysaccharide (PSG-1) in spleen lymphocytes. Our results showed that PSG-1 increased the intracellular Ca2+ concentration and calcineurin (CaN) activity. Moreover, PSG-1 was found to elevate nuclear factor of activated T cells (NFAT) activity, but this effect could be diminished by the treatment of CaN inhibitors (cyclosporin A and FK506). PSG-1-induced interleukin (IL)-2 production was also inhibited by cyclosporin A and FK506. In addition, PSG-1 was found to significantly enhance protein kinase C (PKC) activity. PKC was involved in induction of NFAT activity by PSG-1, as evidenced by abrogation of NFAT activity by PKC inhibitor calphostin C, which significantly decreased PSG-1-induced IL-2 production. On the basis of these results, we concluded that PSG-1 may induce activation of spleen lymphocytes at least in part via the Ca2+/CaN/NFAT/IL-2 signaling pathway and the PKC/NFAT/IL-2 signaling pathway cooperatively regulated PSG-1-induced activation of spleen lymphocytes.
引用
收藏
页码：2734 / 2740
页数：7
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