Contamination of chicken meat with extended-spectrum beta-lactamase producing-Klebsiella pneumoniae and Escherichia coli during scalding and defeathering of broiler carcasses

被引:42
作者
Projahn, Michaela [1 ,5 ]
von Tippelskirch, Philine [2 ]
Semmler, Torsten [3 ]
Guenther, Sebastian [4 ]
Alter, Thomas [2 ]
Roesler, Uwe [1 ]
机构
[1] Free Univ Berlin, Inst Anim Hyg & Environm Hlth, Robert von Ostertag Str 7-13, D-14163 Berlin, Germany
[2] Free Univ Berlin, Inst Food Safety & Food Hyg, Konigsweg 69, D-14163 Berlin, Germany
[3] Robert Koch Inst Berlin, Nordufer 10, D-13353 Berlin, Germany
[4] Ernst Moritz Arndt Univ Greifswald, Inst Pharm, Friedrich Ludwig Jahn Str 17, D-17489 Greifswald, Germany
[5] German Fed Inst Risk Assessment, Diedersdorfer Weg 1, D-12277 Berlin, Germany
关键词
ESBL; Broiler; Contamination; Processing; Enterobacteriaceae; Phylogeny; VIRULENCE-ASSOCIATED GENES; VERTICAL TRANSMISSION; PUBLIC-HEALTH; ENTEROBACTERIACEAE; PREVALENCE; OUTBREAK; RESISTANCE; CLONES; CURLI; CHAIN;
D O I
10.1016/j.fm.2018.09.010
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Extended-spectrum beta-lactamase- (ESBL-) producing Klebsiella (K.) pneumoniae and Escherichia (E.) coli are of critical importance in human and veterinary medicine. Animal food products, especially broiler chickens, are discussed as a possible source for the exposure of humans with antibiotic resistant bacteria. Although the occurrence and vertical transmission of ESBL-/AmpC-producing Enterobacteriaceae in the broiler production has been reported before, detailed investigations concerning the dissemination along the slaughter processing line are missing. In this study, we investigated cross-contamination with ESBL-producing Enterobacteriaceae during the processing of two different broiler flocks in one slaughterhouse. The ESBL-status during the fattening period of the flocks was determined and environmental samples from the slaughterhouse were taken before processing of the respective flocks. These isolates were compared to those found in samples from the carcasses after processing using whole genome sequencing. Phylogenetic analyses of seven ESBL-producing K. pneumoniae and 14 E. coli revealed close relationships between isolates from scalding water and the defeathering machine, respectively, which were collected before the processing of the broiler flocks, to those isolates found in samples from skin and filet of the respective flock carcasses. In conclusion, using high resolution molecular data we found evidence for the cross-contamination of carcasses with ESBL-producing Enterobacteriaceae during scalding and defeathering in the slaughterhouse.
引用
收藏
页码:185 / 191
页数:7
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