Sensitivity of Multiphase Pseudocontinuous Arterial Spin Labelling (MP pCASL) Magnetic Resonance Imaging for Measuring Brain and Tumour Blood Flow in Mice

被引:13
作者
Buck, Jessica [1 ,2 ]
Larkin, James R. [1 ,2 ]
Simard, Manon A. [1 ,2 ]
Khrapitchev, Alexandre A. [1 ,2 ]
Chappell, Michael A. [3 ]
Sibson, Nicola R. [1 ,2 ]
机构
[1] Univ Oxford, Canc Res UK, Oxford OX3 7LE, England
[2] Univ Oxford, Med Res Council Oxford Inst Radiat Oncol, Dept Oncol, Oxford OX3 7LE, England
[3] Univ Oxford, Inst Biomed Engn, Old Rd Campus Res Bldg, Oxford OX3 7DQ, England
基金
英国工程与自然科学研究理事会;
关键词
PERFUSION MRI; AUTORADIOGRAPHY; QUANTIFICATION; INVERSION; VESSELS; CANCER; MODEL; FAIR;
D O I
10.1155/2018/4580919
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Brain and tumour blood flow can be measured noninvasively using arterial spin labelling (ASL) magnetic resonance imaging (MRI), but reliable quantification in mouse models remains difficult. Pseudocontinuous ASL (pCASL) is recommended as the clinical standard for ASL and can be improved using multiphase labelling (MP pCASL). The aim of this study was to optimise and validate MP pCASL MRI for cerebral blood flow (CBF) measurement in mice and to assess its sensitivity to tumour perfusion. Following optimization of the MP pCASL sequence, CBF data were compared with gold-standard autoradiography, showing close agreement. Subsequently, MP pCASL data were acquired at weekly intervals in models of primary and secondary brain tumours, and tumour microvessel density was determined histologically. MP pCASL measurements in a secondary brain tumour model revealed a significant reduction in blood flow at day 35 after induction, despite a higher density of blood vessels. Tumour core regions also showed reduced blood flow compared with the tumour rim. Similarly, significant reductions in CBF were found in a model of glioma 28 days after tumour induction, together with an increased density of blood vessels. These findings indicate that MP pCASL MRI provides accurate and robust measurements of cerebral blood flow in naive mice and is sensitive to changes in tumour perfusion.
引用
收藏
页数:11
相关论文
共 47 条
[41]   Vascular microenvironment in gliomas [J].
Vajkoczy, P ;
Menger, MD .
JOURNAL OF NEURO-ONCOLOGY, 2000, 50 (1-2) :99-108
[42]   Multiple boli arterial spin labeling for high signal-to-noise rodent brain perfusion imaging [J].
Vallatos, Antoine ;
Gilmour, Lesley ;
Chalmers, Anthony J. ;
Holmes, William M. .
MAGNETIC RESONANCE IN MEDICINE, 2018, 79 (02) :1020-1030
[43]   Hypoxia in cancer: significance and impact on clinical outcome [J].
Vaupel, Peter ;
Mayer, Arnulf .
CANCER AND METASTASIS REVIEWS, 2007, 26 (02) :225-239
[44]   Diagnostic performance of spectroscopic and perfusion MRI for distinction of brain tumors [J].
Weber, M. A. ;
Zoubaa, S. ;
Schlieter, M. ;
Juettler, E. ;
Huttner, H. B. ;
Geletneky, K. ;
Ittrich, C. ;
Lichy, M. P. ;
Kroll, A. ;
Debus, J. ;
Giesel, F. L. ;
Hartmann, M. ;
Essig, M. .
NEUROLOGY, 2006, 66 (12) :1899-1906
[45]   MAGNETIC-RESONANCE-IMAGING OF PERFUSION USING SPIN INVERSION OF ARTERIAL WATER [J].
WILLIAMS, DS ;
DETRE, JA ;
LEIGH, JS ;
KORETSKY, AP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (01) :212-216
[46]   Steady Pulsed Imaging and Labeling Scheme for Noninvasive Perfusion Imaging [J].
Xu, Jiadi ;
Qin, Qin ;
Wu, Dan ;
Hua, Jun ;
Song, Xiaolei ;
McMahon, Michael T. ;
Northington, Frances J. ;
Zhang, Jiangyang ;
van Zijl, Peter C. M. ;
Pekar, James J. .
MAGNETIC RESONANCE IN MEDICINE, 2016, 75 (01) :238-248
[47]   High-sensitivity cerebral perfusion mapping in mice by kbGRASE-FAIR at 9.4 T [J].
Zheng, Bingwen ;
Lee, Philip Teck Hock ;
Golay, Xavier .
NMR IN BIOMEDICINE, 2010, 23 (09) :1061-1070