Different effects of valproic acid on proliferation and migration of malignant glioma cells in vitro

被引:0
作者
Knüpfer, MM
Pulzer, F
Schindler, I
Driever, PH
Knüpfer, H
Keller, E
机构
[1] Univ Leipzig, Kinderklin, Paul Flechsig Inst Brain Res, Dept Neuroanat, D-04317 Leipzig, Germany
[2] Univ Leipzig, Childrens Hosp, Dept Neonatol, Leipzig, Germany
[3] Univ Leipzig, Childrens Hosp, Dept Endocrinol, Leipzig, Germany
[4] Humboldt Univ, Univ Hosp, Dept Pediat Haematol & Onocol, Berlin, Germany
关键词
Boyden-chamber; cell culture; spheroid-migration;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Valproic acid (VPA) has been considered as a possible treatment agent for malignant gliomas. In order to characterise the possibilities of VPA, we investigated the effects on cell migration and proliferation. Human cell lines T99G, A172, 85HG66 and 86HG39 were treated with VPA or left untreated, afterwards Boyden chamber assays was used for measuring vertical migration. In a second assay cells were simulated to create spheroids and spheroid migration was measured. Proliferation was assesses using a cell counter. VPA decreased proliferation of 86HG39>A172>85HG66 cells, whereas T98G remained uninfluenced. The influence of VPA of VPA on migration was different; whereas VPA dose-dependently stimulated migration of 86HG39 cells, migration of T98G and 85HG66 decreased, whereas A172 cells remained uninfluenced. Only 86HG39 and A172 cells created spheroids. In both cell lines Boyden-chamber-findings were confirmed by analysing the influence of VPA on spheroid migration. These non-uniform data demonstrate that the benefit of VPA in glioma treatment is not clear and needs further investigation.
引用
收藏
页码:347 / 351
页数:5
相关论文
共 27 条
[1]  
ALBINI A, 1987, CANCER RES, V47, P3239
[2]   MECHANISM OF ANTI-CONVULSANT ACTION OF VALPROATE [J].
CHAPMAN, A ;
KEANE, PE ;
MELDRUM, BS ;
SIMIAND, J ;
VERNIERES, JC .
PROGRESS IN NEUROBIOLOGY, 1982, 19 (04) :315-359
[3]  
CHEN G, 1994, J NEUROCHEM, V63, P2361
[4]   Antitumor activity of sodium valproate in cultures of human neuroblastoma cells [J].
Cinatl, J ;
Cinatl, J ;
Scholz, M ;
Driever, PH ;
Henrich, D ;
Kabickova, H ;
Vogel, JU ;
Doerr, HW ;
Kornhuber, B .
ANTI-CANCER DRUGS, 1996, 7 (07) :766-773
[5]   VALPROIC ACID - A REAPPRAISAL OF ITS PHARMACOLOGICAL PROPERTIES AND CLINICAL EFFICACY IN EPILEPSY [J].
DAVIS, R ;
PETERS, DH ;
MCTAVISH, D .
DRUGS, 1994, 47 (02) :332-372
[6]   TRANSFECTION OF GLIOMA-CELLS WITH THE NEURAL-CELL ADHESION MOLECULE NCAM - EFFECT ON GLIOMA-CELL INVASION AND GROWTH IN-VIVO [J].
EDVARDSEN, K ;
PEDERSEN, PH ;
BJERKVIG, R ;
HERMANN, GG ;
ZEUTHEN, J ;
LAERUM, OD ;
WALSH, FS ;
BOCK, E .
INTERNATIONAL JOURNAL OF CANCER, 1994, 58 (01) :116-122
[7]  
Giese A, 1996, INT J CANCER, V67, P275, DOI 10.1002/(SICI)1097-0215(19960717)67:2<275::AID-IJC20>3.0.CO
[8]  
2-9
[9]   CORRELATES OF SURVIVAL AND THE DAUMAS-DUPORT GRADING SYSTEM FOR ASTROCYTOMAS [J].
KIM, TS ;
HALLIDAY, AL ;
HEDLEYWHYTE, ET ;
CONVERY, K .
JOURNAL OF NEUROSURGERY, 1991, 74 (01) :27-37
[10]  
Knüpfer MM, 1998, ANTICANCER RES, V18, P3585