Drug-Resistant Polymorphisms and Copy Numbers in Plasmodium falciparum, Mozambique, 2015

被引：50

作者：

Gupta, Himanshu ^{[1
]}

Macete, Eusebio ^{[2
]}

Bulo, Helder ^{[2
]}

Salvador, Crizolgo ^{[3
]}

Warsame, Marian ^{[4
]}

Carvalho, Eva ^{[5
]}

Menard, Didier ^{[6
,7
]}

Ringwald, Pascal ^{[4
]}

Bassat, Quique ^{[1
,2
,8
,9
]}

Enosse, Sonia ^{[3
]}

Mayor, Alfredo ^{[1
,2
]}

机构：

[1] Univ Barcelona, Hosp Clin, Barcelona Inst Global Hlth, ISGlobal, Barcelona, Spain

[2] Ctr Invest Saude Manhica, Maputo, Mozambique

[3] Minist Saude, Inst Nacl Saude, Maputo, Mozambique

[4] WHO, Global Malaria Programme, Geneva, Switzerland

[5] WHO, Maputo, Mozambique

[6] Inst Pasteur Cambodge, Malaria Mol Epidemiol Unit 16, Phnom Penh, Cambodia

[7] Inst Pasteur, Paris, France

[8] ICREA, Barcelona, Spain

[9] Univ Barcelona, Hosp St Joan de Deu, Pediat Dept, Barcelona, Spain

来源：

EMERGING INFECTIOUS DISEASES | 2018年 / 24卷 / 01期

关键词：

DIHYDROARTEMISININ-PIPERAQUINE; ARTEMETHER-LUMEFANTRINE; ARTEMISININ RESISTANCE; PFMDR1; GENE; K13-PROPELLER POLYMORPHISMS; WESTERN CAMBODIA; MALARIA; CHLOROQUINE; MEFLOQUINE; EFFICACY;

D O I：

10.3201/eid2401.170864

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

One of the fundamental steps toward malaria control is the use of antimalarial drugs. The success of antimalarial treatment can be affected by the presence of drug-resistant populations of Plasmodium falciparum. To assess resistance, we used molecular methods to examine 351 P. falciparum isolates collected from 4 sentinel sites in Mozambique for K13, pfmdr1, pfcrt, and pfdhps polymorphisms and for plasmepsin2 (pfpm2) and pfmdr1 copy numbers. We found multiple copies of pfpm2 in 1.1% of isolates. All isolates carried K13 wild-type alleles (3D7-like), except 4 novel polymorphisms (Leu619Leu, Phe656Ile, Val666Val, Gly690Gly). Prevalence of isolates with pfcrt mutant (K76T) allele was low (2.3%). Prevalence of isolates with pfdhps mutant alleles (A437G and K540E) was > 80%, indicating persistence of sulfadoxine/pyrimethamine resistance; however, markers of artemisinin were absent, and markers of piperaquine resistance were low. Piperaquine resistance isolates may spread in Mozambique as dihydroartemisinin/piperaquine drug pressure increases.

引用

页码：40 / 48

页数：9

共 50 条

[31] Pfmdr1 copy number and arteminisin derivatives combination therapy failure in falciparum malaria in Cambodia [J].

Lim, Pharath ;

Alker, Alisa P. ;

Khim, Nimol ;

Shah, Naman K. ;

Incardona, Sandra ;

Doung, Socheat ;

Yi, Poravuth ;

Bouth, Denis Mey ;

Bouchier, Christiane ;

Puijalon, Odile Mercereau ;

Meshnick, Steven R. ;

Wongsrichanalai, Chansuda ;

Fandeur, Thierry ;

Le Bras, Jacques ;

Ringwald, Pascal ;

Ariey, Frederic .

MALARIA JOURNAL, 2009, 8

[32] Sub-microscopic infections and long-term recrudescence of Plasmodium falciparum in Mozambican pregnant women [J].

Mayor, Alfredo ;

Serra-Casas, Elisa ;

Bardaji, Azucena ;

Sanz, Sergi ;

Puyol, Laura ;

Cistero, Pau ;

Sigauque, Betuel ;

Mandomando, Inacio ;

Aponte, John J. ;

Alonso, Pedro L. ;

Menendez, Clara .

MALARIA JOURNAL, 2009, 8

[33] A Worldwide Map of Plasmodium falciparum K13-Propeller Polymorphisms [J].

Menard, D. ;

Khim, N. ;

Beghain, J. ;

Adegnika, A. A. ;

Shafiul-Alam, M. ;

Amodu, O. ;

Rahim-Awab, G. ;

Barnadas, C. ;

Berry, A. ;

Boum, Y. ;

Bustos, M. D. ;

Cao, J. ;

Chen, J. -H. ;

Collet, L. ;

Cui, L. ;

Thakur, G. -D. ;

Dieye, A. ;

Djalle, D. ;

Dorkenoo, M. A. ;

Eboumbou-Moukoko, C. E. ;

Espino, F. -E. -C. J. ;

Fandeur, T. ;

Ferreira-da-Cruz, M. -F ;

Fola, A. A. ;

Fuehrer, H-P. ;

Hassan, A. M. ;

Herrera, S. ;

Hongvanthong, B. ;

Houze, S. ;

Ibrahim, M. L. ;

Jahirul-Karim, M. ;

Jiang, L. ;

Kano, S. ;

Ali-Khan, W. ;

Khanthavong, M. ;

Kremsner, P. G. ;

Lacerda, M. ;

Leang, R. ;

Leelawong, M. ;

Li, M. ;

Lin, K. ;

Mazarati, J. -B. ;

Menard, S. ;

Morlais, I. ;

Muhindo-Mavoko, H. ;

Musset, L. ;

Na-Bangchang, K. ;

Nambozi, M. ;

Niare, K. ;

Noedl, H. .

NEW ENGLAND JOURNAL OF MEDICINE, 2016, 374 (25) :2453-2464

[34] Genetic architecture of artemisinin-resistant Plasmodium falciparum [J].

Miotto, Olivo ;

Amato, Roberto ;

Ashley, Elizabeth A. ;

MacInnis, Bronwyn ;

Almagro-Garcia, Jacob ;

Amaratunga, Chanaki ;

Lim, Pharath ;

Mead, Daniel ;

Oyolal, Samuel O. ;

Dhorda, Mehul ;

Imwong, Mallika ;

Woodrow, Charles ;

Manske, Magnus ;

Stalker, Jim ;

Drury, Eleanor ;

Campino, Susana ;

Amenga-Etego, Lucas ;

Thuy-Nhien Nguyen Thanh ;

Hien Tinh Tran ;

Ringwald, Pascal ;

Bethellm, Delia ;

Nosten, Francois ;

Phyo, Aung Pyae ;

Pukrittayakamee, Sasithon ;

Chotivanich, Kesinee ;

Chuor, Char Meng ;

Nguon, Chea ;

Suon, Seila ;

Sreng, Sokunthea ;

Newton, Paul N. ;

Mayxay, Mayfong ;

Khanthavong, Maniphone ;

Hongvanthong, Bouasy ;

Htut, Ye ;

Han, Kay Thwe ;

Kyaw, Myat Phone ;

Faiz, Md Abul ;

Fanello, Caterina I. ;

Onyamboko, Marie ;

Mokuolu, Olugbenga A. ;

Jacob, Christopher G. ;

Takala-Harrison, Shannon ;

Plowe, Christopher V. ;

Day, Nicholas P. ;

Dondorp, Arjen M. ;

Spencer, Chris C. A. ;

McVean, Gilean ;

Fairhurst, Rick M. ;

White, Nicholas J. ;

Kwiatkowski, Dominic P. .

NATURE GENETICS, 2015, 47 (03) :226-+

[35] Evidence of Artemisinin-Resistant Malaria in Western Cambodia [J].

Noedl, Harald ;

Se, Youry ;

Schaecher, Kurt ;

Smith, Bryan L. ;

Socheat, Duong ;

Fukuda, Mark M. .

NEW ENGLAND JOURNAL OF MEDICINE, 2008, 359 (24) :2619-2620

[36] Changes in the burden of malaria in sub-Saharan Africa [J].

O'Meara, Wendy Prudhomme ;

Mangeni, Judith Nekesa ;

Steketee, Rick ;

Greenwood, Brian .

LANCET INFECTIOUS DISEASES, 2010, 10 (08) :545-555

[37] Drug-resistant malaria: Molecular mechanisms and implications for public health [J].

Petersen, Ines ;

Eastman, Richard ;

Lanzer, Michael .

FEBS LETTERS, 2011, 585 (11) :1551-1562

[38] The pfmdr1 gene is associated with a multidrug-resistant phenotype in Plasmodium falciparum from the western border of Thailand [J].

Price, RN ;

Cassar, C ;

Brockman, A ;

Duraisingh, M ;

van Vugt, M ;

White, NJ ;

Nosten, F ;

Krishna, S .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (12) :2943-2949

[39] Mefloquine resistance in Plasmodium falciparum and increased pfmdr1 gene copy number [J].

Price, RN ;

Uhlemann, AC ;

Brockman, A ;

McGready, R ;

Ashley, E ;

Phaipun, L ;

Patel, R ;

Laing, K ;

Looareesuwan, S ;

White, NJ ;

Nosten, F ;

Krishna, S .

LANCET, 2004, 364 (9432) :438-447

[40] Five Years of Antimalarial Resistance Marker Surveillance in Gaza Province, Mozambique, Following Artemisinin-Based Combination Therapy Roll Out [J].

Raman, Jaishree ;

Mauff, Katya ;

Muianga, Pedro ;

Mussa, Abdul ;

Maharaj, Rajendra ;

Barnes, Karen I. .

PLOS ONE, 2011, 6 (10)

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