Drug-Resistant Polymorphisms and Copy Numbers in Plasmodium falciparum, Mozambique, 2015

被引：50

作者：

Gupta, Himanshu ^{[1
]}

Macete, Eusebio ^{[2
]}

Bulo, Helder ^{[2
]}

Salvador, Crizolgo ^{[3
]}

Warsame, Marian ^{[4
]}

Carvalho, Eva ^{[5
]}

Menard, Didier ^{[6
,7
]}

Ringwald, Pascal ^{[4
]}

Bassat, Quique ^{[1
,2
,8
,9
]}

Enosse, Sonia ^{[3
]}

Mayor, Alfredo ^{[1
,2
]}

机构：

[1] Univ Barcelona, Hosp Clin, Barcelona Inst Global Hlth, ISGlobal, Barcelona, Spain

[2] Ctr Invest Saude Manhica, Maputo, Mozambique

[3] Minist Saude, Inst Nacl Saude, Maputo, Mozambique

[4] WHO, Global Malaria Programme, Geneva, Switzerland

[5] WHO, Maputo, Mozambique

[6] Inst Pasteur Cambodge, Malaria Mol Epidemiol Unit 16, Phnom Penh, Cambodia

[7] Inst Pasteur, Paris, France

[8] ICREA, Barcelona, Spain

[9] Univ Barcelona, Hosp St Joan de Deu, Pediat Dept, Barcelona, Spain

来源：

EMERGING INFECTIOUS DISEASES | 2018年 / 24卷 / 01期

关键词：

DIHYDROARTEMISININ-PIPERAQUINE; ARTEMETHER-LUMEFANTRINE; ARTEMISININ RESISTANCE; PFMDR1; GENE; K13-PROPELLER POLYMORPHISMS; WESTERN CAMBODIA; MALARIA; CHLOROQUINE; MEFLOQUINE; EFFICACY;

D O I：

10.3201/eid2401.170864

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

One of the fundamental steps toward malaria control is the use of antimalarial drugs. The success of antimalarial treatment can be affected by the presence of drug-resistant populations of Plasmodium falciparum. To assess resistance, we used molecular methods to examine 351 P. falciparum isolates collected from 4 sentinel sites in Mozambique for K13, pfmdr1, pfcrt, and pfdhps polymorphisms and for plasmepsin2 (pfpm2) and pfmdr1 copy numbers. We found multiple copies of pfpm2 in 1.1% of isolates. All isolates carried K13 wild-type alleles (3D7-like), except 4 novel polymorphisms (Leu619Leu, Phe656Ile, Val666Val, Gly690Gly). Prevalence of isolates with pfcrt mutant (K76T) allele was low (2.3%). Prevalence of isolates with pfdhps mutant alleles (A437G and K540E) was > 80%, indicating persistence of sulfadoxine/pyrimethamine resistance; however, markers of artemisinin were absent, and markers of piperaquine resistance were low. Piperaquine resistance isolates may spread in Mozambique as dihydroartemisinin/piperaquine drug pressure increases.

引用

页码：40 / 48

页数：9

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