Production of Methionol from 3-Methylthiopropionaldehyde by Catalysis of the Yeast Alcohol Dehydrogenase Adh4p

被引：18

作者：

Che, Yixin ^{[1
,2
]}

Yin, Sheng ^{[1
,2
]}

Wang, Hui ^{[1
,2
]}

Yang, Huaqing ^{[1
,2
]}

Xu, Ruixin ^{[1
,2
]}

Wang, Qi ^{[1
,2
]}

Wu, Yiping ^{[1
,2
]}

Boutet, Julien ^{[3
,4
]}

Huet, Robert ^{[3
,4
]}

Wang, Chengtao ^{[1
,2
]}

机构：

[1] Beijing Technol & Business Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing Engn & Technol Res Ctr Food Additives, Beijing 100048, Peoples R China

[2] Beijing Technol & Business Univ, Sch Food & Hlth, Beijing 100048, Peoples R China

[3] Adisseo France SAS, F-92160 Antony, France

[4] Bluestar Adisseo Nanjing Co Ltd, Nanjing 210047, Jiangsu, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2020年 / 68卷 / 16期

基金：

北京市自然科学基金; 中国国家自然科学基金;

关键词：

Methionol; 3-Methylthiopropionaldehyde; Alcohol dehydrogenase; ADH4; Saccharomyces cerevisiae; SULFUR-COMPOUNDS; ESCHERICHIA-COLI; OPTIMIZATION; PURIFICATION; METABOLISM; EXPRESSION;

D O I：

10.1021/acs.jafc.0c00776

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Methionol is a sulfur-containing aroma compound that contributes to the flavors of fermented foods. In this work, a novel method for methionol production was established using 3-methylthiopropionaldehyde (MMP) and alcohol dehydrogenase (ADH). First, expression of seven ADH genes was analyzed in yeast fermentation added with MMP. Only ADH4 displayed an evident increased expression in response to MMP. ADH4 was then overexpressed in Saccharomyces cerevisiae S288c and Escherichia coli BL21. The recombinant yeast strain S4 produced more methionol than control strain in MMP fermentation. Furthermore, 0.55 g/L 42 kDa Adh4p was prepared from E. coli by induced expression and purification. A fed-batch catalysis system was finally established to produce methionol from MMP by Adh4p. In 10 h of continuous catalysis, the conversion rate of MMP remained 80-95%, and a final yield of 0.2 g/L methionol was achieved. This work proposed a novel method for methionol production by enzymatic catalysis with a potential application prospect in industry.

引用

页码：4650 / 4656

页数：7

共 29 条

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