Ancient bacterial DNA (aDNA) in dental calculus from archaeological human remains

被引：43

作者：

Preus, Hans R. ^{[1
]}

Marvik, Ole J. ^{[2
]}

Selvig, Knut A. ^{[3
]}

Bennike, Pia ^{[4
]}

机构：

[1] Univ Oslo, Fac Dent, Dept Periodontol, Inst Clin Odontol, N-0317 Oslo, Norway

[2] Affitech AS, Oslo, Norway

[3] Univ Bergen, Fac Dent, Dept Anat, N-5020 Bergen, Norway

[4] Univ Copenhagen, Saxo Inst, DK-1168 Copenhagen, Denmark

来源：

JOURNAL OF ARCHAEOLOGICAL SCIENCE | 2011年 / 38卷 / 08期

关键词：

Bacteria; Dental calculus; Transmission electron microscopy; Bioarchaeology; Immunolabeling; PERIODONTAL-DISEASE; OPAL PHYTOLITHS; PCR; CONTAMINATION;

D O I：

10.1016/j.jas.2011.03.020

中图分类号：

Q98 [人类学];

学科分类号：

030303 ;

摘要：

The purpose of this study was to identify reactive bacterial aDNA in archaeological human dental calculus. Dental calculus was collected from a middle/late Neolithic human skull from Hulbjerg passage grave, Langeland, Denmark and prepared for transmission electron microscopy (TEM) or gold-labeled antibody TEM. TEM showed calcified, as well as non-calcified bacteria. Immunogold labeling occurred over the cytoplasmic portions of the sectioned bacteria. The result demonstrated that it is possible to identify aDNA sequences from bacteria in archaeological material of considerable age by this technique. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1827 / 1831

页数：5

共 39 条

[1]

[Anonymous], 1995, Z MORPHOLOGIE ANTHR

[2]

[Anonymous], PRZ ANTROPOL ANTHR R

[3]

Bennike P., 1990, Proceedings from Osteoporosis 1990, P89

[4]

Bennike P., 1986, Bulletin of the History of Dentistry, V34, P81

[5]

Bennike Pia, 1985, YNGRE STENALDER OERN, P467

[6] Temperature monitoring in archaeological animal bone samples in the Near East and area, before, during and after excavation [J].

Bollongino, Ruth ;

Vigne, Jean-Denis .

JOURNAL OF ARCHAEOLOGICAL SCIENCE, 2008, 35 (04) :873-881

[7] Brief communication: Identification of the authentic ancient DNA sequence in a human bone contaminated with modern DNA [J].

Bouwman, Abigail S. ;

Chilvers, Elizabeth R. ;

Brown, Keri A. ;

Brown, Terence A. .

AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY, 2006, 131 (03) :428-431

[8]

Burger J, 1997, Anthropol Anz, V55, P193

[9]

Campana M. G., 2008, ARCHAEOLOGICAL REV C, V23. 2, P147

[10] DIFFERING PROFILES OF PERIODONTAL-DISEASE IN 2 SIMILAR SOUTH-PACIFIC ISLAND POPULATIONS [J].

CUTRESS, TW ;

POWELL, RN ;

BALL, ME .

COMMUNITY DENTISTRY AND ORAL EPIDEMIOLOGY, 1982, 10 (04) :193-203

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