Effect of SOS-induced levels of imuABC on spontaneous and damage-induced mutagenesis in Caulobacter crescentus

被引：14

作者：

Alves, Ingrid R. ^{[1
]}

Lima-Noronha, Marco A. ^{[1
]}

Silva, Larissa G. ^{[1
]}

Fernandez-Silva, Frank S. ^{[1
]}

Freitas, Aline Luiza D. ^{[1
]}

Marques, Marilis V. ^{[1
]}

Galhardo, Rodrigo S. ^{[1
]}

机构：

[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, SP, Brazil

来源：

DNA REPAIR | 2017年 / 59卷

基金：

巴西圣保罗研究基金会;

关键词：

imuABC; dnaE2; recA; Caulobacter crescentus; SOS response; DNA-POLYMERASE V; ESCHERICHIA-COLI; UNTARGETED MUTAGENESIS; ANTIBIOTIC-RESISTANCE; ERROR-PRONE; POL-V; MUTATION; RECA; REPLICATION; INVOLVEMENT;

D O I：

10.1016/j.dnarep.2017.09.003

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

imuABC (brtuAB dnaE2) genes are responsible for SOS-mutagenesis in Caulobacter crescentus and other bacterial species devoid of umuDC. In this work, we have constructed operator-constitutive mutants of the imuABC operon. We used this genetic tool to investigate the effect of SOS-induced levels of these genes upon both spontaneous and damage-induced mutagenesis. We showed that constitutive expression of imuABC does not increase spontaneous or damage-induced mutagenesis, nor increases cellular resistance to DNA-damaging agents. Nevertheless; the presence of the operator-constitutive mutation rescues mutagenesis in a recA background, indicating that imuABC are the only genes required at SOS-induced levels for translesion synthesis (TLS) in C. crescentus. Furthermore, these data also show that TLS mediated by ImuABC does not require RecA, unlike umuDC-dependent mutagenesis in E. coli.

引用

页码：20 / 26

页数：7

共 43 条

[1] RecA: peculation and Mechanism of a Molecular Search Engine
Bell, Jason C.
Kowalczykowski, Stephen C.
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 2016, 41 (07) : 646 - 646
[2] DnaE2 polymerase contributes to in vivo survival and the emergence of drug resistance in Mycobacterium tuberculosis
Boshoff, HIM
Reed, MB
Barry, CE
Mizrahi, V
[J]. CELL, 2003, 113 (02) : 183 - 193
[3] The essential C family DnaE polymerase is error-prone and efficient at lesion bypass
Bruck, I
Goodman, MF
O'Donnell, M
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (45) : 44361 - 44368
[4] NATURE OF THE SOS MUTATOR ACTIVITY - GENETIC-CHARACTERIZATION OF UNTARGETED MUTAGENESIS IN ESCHERICHIA-COLI
CAILLETFAUQUET, P
MAENHAUTMICHEL, G
[J]. MOLECULAR & GENERAL GENETICS, 1988, 213 (2-3): : 491 - 498
[5] UNTARGETED MUTAGENESIS INDUCED BY UV IN THE IACI GENE OF ESCHERICHIA-COLI
CHRISTENSEN, RB
CHRISTENSEN, JR
KOENIG, I
LAWRENCE, CW
[J]. MOLECULAR AND GENERAL GENETICS, 1985, 201 (01): : 30 - 34
[6] Inhibition of mutation and combating the evolution of antibiotic resistance
Cirz, RT
Chin, JK
Andes, DR
de Crécy-Lagard, V
Craig, WA
Romesberg, FE
[J]. PLOS BIOLOGY, 2005, 3 (06) : 1024 - 1033
[7] Characterization of the SOS regulon of Caulobacter crescentus
da Rocha, Raquel Paes
Paquola, Apua Cesar De Miranda
Marques, Marilis do Valle
Menck, Carlos Frederico Martins
Galhardo, Rodrigo S.
[J]. JOURNAL OF BACTERIOLOGY, 2008, 190 (04) : 1209 - 1218
[8] ANALYSIS OF RECA MUTANTS WITH ALTERED SOS FUNCTIONS
ENNIS, DG
LEVINE, AS
KOCH, WH
WOODGATE, R
[J]. MUTATION RESEARCH-DNA REPAIR, 1995, 336 (01): : 39 - 48
[9] ENVELOPE-ASSOCIATED NUCLEOID FROM CAULOBACTER-CRESCENTUS STALKED AND SWARMER CELLS
EVINGER, M
AGABIAN, N
[J]. JOURNAL OF BACTERIOLOGY, 1977, 132 (01) : 294 - 301
[10] Biochemical basis for the essential genetic requirements of RecA and the β-clamp in Pol V activation
Fujii, Shingo
Fuchs, Robert P.
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (35) : 14825 - 14830

← 1 2 3 4 5 →