Assessment of Cardiovascular Fibrosis Using Novel Fluorescent Probes

被引：25

作者：

Chen, Jiqiu ^{[1
]}

Lee, Seung Koo ^{[2
]}

Abd-Elgaliel, Wael R. ^{[2
]}

Liang, Lifan ^{[1
]}

Galende, Elisa-Yaniz ^{[1
]}

Hajjar, Roger J. ^{[1
]}

Tung, Ching-Hsuan ^{[2
]}

机构：

[1] Mt Sinai Sch Med, Cardiovasc Res Ctr, New York, NY USA

[2] Cornell Univ, Methodist Hosp, Weill Med Coll, Dept Radiol,Res Inst, Houston, TX USA

来源：

PLOS ONE | 2011年 / 6卷 / 04期

基金：

美国国家卫生研究院;

关键词：

HEART-FAILURE; DIASTOLIC DYSFUNCTION; GENE-TRANSFER; HUG MODEL; COLLAGEN; HYPERTROPHY; BINDING; MECHANISMS; DISEASE;

D O I：

10.1371/journal.pone.0019097

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cardiovascular fibrosis resulted from pressure overload or ischemia could alter myocardial stiffness and lead to ventricular dysfunction. Fluorescently labeled collagen-binding protein CNA 35, derived from the surface component of Staphylococcus aureus, and a novel synthetic biphenylalanine containing peptide are applied to stain fibrosis associated collagen and myocytes, respectively. Detailed pathological characteristics of cardiovascular fibrosis could be identified clearly in 2 hours. This staining pair requires only simple staining and brief washing, generating less than 10 ml of waste. The image information collected by this novel fluorescent staining pair is compatible with it collected by the traditional Masson's Trichrome and Picrosirius Red staining which are widely used to stain cardiovascular fibrosis and isolated cells.

引用

页数：6

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