Schistosomicidal evaluation of flavonoids from two species of Styrax against Schistosoma mansoni adult worms

被引:29
作者
Braguine, Caio Guedes [1 ]
Bertanha, Camila Spereta [1 ]
Goncalves, Ubirajara Oliveira [1 ]
Magalhaes, Lizandra Guidi [1 ]
Rodrigues, Vanderlei [2 ]
Melleiro Gimenez, Valeria Maria [3 ]
Groppo, Milton [4 ]
Andrade e Silva, Marcio Luis [1 ]
Cunha, Wilson Roberto [1 ]
Januario, Ana Helena [1 ]
Pauletti, Patricia Mendonca [1 ]
机构
[1] Univ Franca, BR-14404600 Franca, SP, Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Ribeirao Preto, SP, Brazil
[3] Ctr Univ Claretiano, Batatais, SP, Brazil
[4] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Pret, Ribeirao Preto, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
Flavonol; schistosomicidal activity; Styrax camporum; Styrax pohlii; CONSTITUENTS; CUPULIFERAE; INHIBITORS; LIGNANS;
D O I
10.3109/13880209.2011.649857
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Context: Schistosomiasis is a major health problem worldwide. Thus, the search for new schistosomicidal agents from natural sources can provide prototypes for drug discovery. Objective: The present study investigated the chemical composition of the EtOAc fractions of Styrax pohlii Pohl (Styracaceae) (EF-SP) aerial parts and S. camporum A. DC. leaves (EF-SC), as well as schistosomicidal activities against Schistosoma mansoni adult worms, which have not yet been studied. Materials and methods: The crude ethanol extracts of S. camporum leaves and S. pohlii aerial parts (EE-SC and EE-SP) were partitioned with n-hexane, EtOAc, and n-BuOH. The EtOAc fractions were purified by preparative HPLC. The crude extracts, EtOAc fractions and pure compounds were tested against S. mansoni adult worms in vitro. Results: The purification procedure resulted in the isolation of kaempferol-3-O-(2 '',4 ''-di-O-(E)-p-coumaroyl)-beta-D-glucopyranoside (1), kaempferol-3-O-(2 '',6 ''-di-O-(E)-p-coumaroyl)-beta-D-glucopyranoside (2), quercetin (3), and kaempferol (4). The bioassay results indicated that EE-SC, EF-SC, EF-SP, and compounds 2 and 4 are able to separate coupled S. mansoni adult worms. Additionally, EE-SC, EF-SP, and compound 4 killed the adult schistosomes in vitro at 100 mu g/mL and 100 mu M. Discussion and conclusion: This is the first time that the presence of compounds 1-2 in S. pohlii and 3-4 in S. camporum has been reported. Additionally, biological results indicated that S. pohlii and S. camporum have great potential as a source of active compounds.
引用
收藏
页码:925 / 929
页数:5
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