Expression profile analysis of microRNAs and downregulated miR-486-5p and miR-30a-5p in non-small cell lung cancer

被引:68
作者
Zhu, Jianjie [1 ,2 ]
Zeng, Yuanyuan [1 ,2 ]
Xu, Chun [3 ]
Qin, Hualong [3 ]
Lei, Zhe [4 ]
Shen, Dan [1 ]
Liu, Zeyi [1 ,2 ,4 ]
Huang, Jian-An [1 ,2 ]
机构
[1] Soochow Univ, Affiliated Hosp 1, Dept Resp Med, Suzhou 215123, Jiangsu, Peoples R China
[2] Soochow Univ, Inst Resp Dis, Suzhou 215123, Jiangsu, Peoples R China
[3] Soochow Univ, Affiliated Hosp 1, Dept Cardiothorac Surg, Suzhou 215123, Jiangsu, Peoples R China
[4] Suzhou Key Lab Mol Canc Genet, Suzhou, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
microRNAs; signaling pathway; non-small cell lung cancer; MESENCHYMAL TRANSITION; BREAST-CANCER; GENE ONTOLOGY; DIAGNOSIS; SPUTUM; PROLIFERATION; PROGRESSION; METASTASIS; SIGNATURE; CARCINOMA;
D O I
10.3892/or.2015.4141
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Lung cancer is the leading cause of cancer-related mortality worldwide and although there have been improvements in treatment there is a low survival rate. The aim of the present study was to investigate the effect of microRNA (miRNA) on cell pathways. A miRNA microarray was used to profile miRNAs of lung cancer tissues. It was identified that 33 miRNAs with >2.0-fold change and FDR <0.05 were differentially expressed between the adjacent non-cancerous lung tissues and non-small cell lung cancers NSCLCs (P<0.005). The data were optimized in combination with physical interaction analysis to obtain crucial miRNAs. The results showed that differentially expressed miRNAs were associated with biological processes such as cell migration, protein phosphorylation and neuron differentiation, and signaling pathways such as MAPK, TGF-(3 and PI3K/Akt signaling pathways. Validation of significant miRNAs in independent 40 paired NSCLC tissues demonstrated that the expression level of miR-486-5p and miR-30a-5p was significantly downregulated in another 40 paired lung cancer tissues. Taken together, the results provided strong evidence of the possible involvement of miRNAs in the development and progression of NSCLC. Thus, the results are of importance for clinical investigators and for those who design miRNA-based novel cancer therapeutics.
引用
收藏
页码:1779 / 1786
页数:8
相关论文
共 45 条
[1]   The miR-30 miRNA family regulates Xenopus pronephros development and targets the transcription factor Xlim1/Lhx1 [J].
Agrawal, Raman ;
Tran, Uyen ;
Wessely, Oliver .
DEVELOPMENT, 2009, 136 (23) :3927-3936
[2]   Gene Ontology: tool for the unification of biology [J].
Ashburner, M ;
Ball, CA ;
Blake, JA ;
Botstein, D ;
Butler, H ;
Cherry, JM ;
Davis, AP ;
Dolinski, K ;
Dwight, SS ;
Eppig, JT ;
Harris, MA ;
Hill, DP ;
Issel-Tarver, L ;
Kasarskis, A ;
Lewis, S ;
Matese, JC ;
Richardson, JE ;
Ringwald, M ;
Rubin, GM ;
Sherlock, G .
NATURE GENETICS, 2000, 25 (01) :25-29
[3]   Promoter hypermethylation of multiple genes in sputum precedes lung cancer incidence in a high-risk cohort [J].
Belinsky, SA ;
Liechty, KC ;
Gentry, FD ;
Wolf, HJ ;
Rogers, J ;
Vu, K ;
Haney, J ;
Kenned, TC ;
Hirsch, FR ;
Miller, Y ;
Franklin, WA ;
Herman, JG ;
Baylin, SB ;
Bunn, PA ;
Byers, T .
CANCER RESEARCH, 2006, 66 (06) :3338-3344
[4]   MicroRNA-205 signaling regulates mammary stem cell fate and tumorigenesis [J].
Chao, Chi-Hong ;
Chang, Chao-Ching ;
Wu, Meng-Ju ;
Ko, How-Wen ;
Wang, Da ;
Hung, Mien-Chie ;
Yang, Jer-Yen ;
Chang, Chun-Ju .
JOURNAL OF CLINICAL INVESTIGATION, 2014, 124 (07) :3093-3106
[5]   A systems biology approach for pathway level analysis [J].
Draghici, Sorin ;
Khatri, Purvesh ;
Tarca, Adi Laurentiu ;
Amin, Kashyap ;
Done, Arina ;
Voichita, Calin ;
Georgescu, Constantin ;
Romero, Roberto .
GENOME RESEARCH, 2007, 17 (10) :1537-1545
[6]   Genomic profiles in stage I primary non small cell lung cancer using comparative genomic hybridization analysis of cDNA microarrays [J].
Feng, J ;
Yin, ZN ;
Caraway, NP ;
Li, RY ;
Katz, RL .
NEOPLASIA, 2004, 6 (05) :623-635
[7]   The mir-200 family and mir-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1 [J].
Gregory, Philip A. ;
Bert, Andrew G. ;
Paterson, Emily L. ;
Barry, Simon C. ;
Tsykin, Anna ;
Farshid, Gelareh ;
Vadas, Mathew A. ;
Khew-Goodall, Yeesim ;
Goodall, Gregory J. .
NATURE CELL BIOLOGY, 2008, 10 (05) :593-601
[8]   Mammalian microRNAs predominantly act to decrease target mRNA levels [J].
Guo, Huili ;
Ingolia, Nicholas T. ;
Weissman, Jonathan S. ;
Bartel, David P. .
NATURE, 2010, 466 (7308) :835-U66
[9]   Copy number variation at 6q13 is associated with lung cancer risk in a Han Chinese population [J].
Hu, Xiao-Yun ;
Bai, Xu-Ming ;
Qiao, Xue ;
Zhu, Ya-Qun .
EXPERIMENTAL LUNG RESEARCH, 2013, 39 (10) :427-433
[10]   Combined genetic analysis of sputum and computed tomography for noninvasive diagnosis of non-small-cell lung cancer [J].
Jiang, Feng ;
Todd, Nevins W. ;
Qiu, Qi ;
Liu, Zhenqiu ;
Katz, Ruth L. ;
Stass, Sanford A. .
LUNG CANCER, 2009, 66 (01) :58-63