Intracellular localization of the tumor suppressor HtrA1/Prss11 and its association with HPV16 E6 and E7 proteins

被引：32

作者：

Clawson, Gary A. ^{[1
,2
,3
]}

Bui, Vuong ^{[1
,3
]}

Xin, Ping ^{[1
,3
]}

Wang, Ning ^{[1
,3
]}

Pan, Weihua ^{[1
,3
]}

机构：

[1] Penn State Univ, Milton S Hershey Med Ctr, Gittlen Canc Res Fdn, Dept Pathol, Hershey, PA 17033 USA

[2] Mat Res Inst, University Pk, PA USA

[3] Penn State Univ, Milton S Hershey Med Ctr, Gittlen Canc Res Fdn, Dept Biochem & Mol Biol, Hershey, PA 17033 USA

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2008年 / 105卷 / 01期

关键词：

HtrA1; Prss11; HPV; cancer;

D O I：

10.1002/jcb.21804

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have a long-standing interest in a nuclear protease which appears to be involved in carcinogenesis. We recently identified the protease as high temperature requirement factor A 1 (HtrA1), also known as Prss11, which is member of an oxidative stress-response family of proteases. HtrA1 has been classified as a secreted protease involved in TGF beta signaling, but recent work has shown HtrA1 to be a tumor suppressor. Here we show that: processed forms of HtrA1 are found intracellularly and intranuclearly, and the active intranuclear form of HtrA1 shows an similar to Mr 29,000. Further, expression of HPV E6/E7 proteins is associated with a post-transcriptional up-regulation of HtrA1 (most notably the nuclear form), and HtrA1 is found associated with both HPV E6 and E7 proteins.

引用

页码：81 / 88

页数：8

共 28 条

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