Structure and nucleotide polymorphisms in pig uncoupling protein 2 and 3 genes

被引：3

作者：

Li, HJ ^{[1
]}

Li, YH ^{[1
]}

Zhao, XB ^{[1
]}

Li, N ^{[1
]}

Wu, CX ^{[1
]}

机构：

[1] China Agr Univ, Coll Anim Sci & Technol, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China

来源：

ANIMAL BIOTECHNOLOGY | 2005年 / 16卷 / 02期

关键词：

pig; UCP2; UCP3; polymorphism; second transmembrane domain;

D O I：

暂无

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Uncoupling proteins (UCPs) are mitochondrial membrane transporters, acting as an uncoupler in oxidative phosphorylation. In this study, we designed 11 primer sets based on the human and mouse UCP2, UCP3 sequences and successfully amplified full regions of porcine UCP2 and UCP3 by polymerase chain reactions (PCR). Comparison of the UCP2 and UCP3 genic structures revealed a highly conservative region was putatively presented, showing the second transmembrane domain may be the UCPs' cardinal function region. Altogether 23 nucleotide polymorphisms of UCP2 and UCP3 genes were discovered in Yorkshire, Wuzhishan, and Lepinghua pigs. These polymorphisms included 3 missense mutations, 16 intronic substitutions, and 4 intronic deletions. The substitution of Ala-55-Val in UCP2 is actually the most common mutation in human. We also calculated genotypic frequencies of five polymorphisms in three pig breeds.

引用

页码：209 / 220

页数：12

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