Preparative separation of dryofragin and aspidin BB from Dryopteris fragrans extracts by macroporous resin column chromatography

被引：51

作者：

Li, Xiao-Juan ^{[1
,2
]}

Fu, Yu-Jie ^{[1
,2
]}

Luo, Meng ^{[1
,2
]}

Wang, Wei ^{[1
,2
]}

Zhang, Lin ^{[1
,2
]}

Zhao, Chun-Jian ^{[1
,2
]}

Zu, Yuan-Gang ^{[1
,2
]}

机构：

[1] NE Forestry Univ, Key Lab Forest Plant Ecol, Minist Educ, Harbin 150040, Peoples R China

[2] NE Forestry Univ, Engn Res Ctr Forest Biopreparat, Minist Educ, Harbin 150040, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2012年 / 61卷

关键词：

Aspidin BB; Column chromatography; Dryofragin; Macroporous resins; Separation; PHLOROGLUCINOL DERIVATIVES; PURIFICATION; PROTEIN;

D O I：

10.1016/j.jpba.2011.12.003

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple, efficient and environment-friendly chromatographic separation method was developed for preparative separation and enrichment of dryofragin and aspidin BB from Dryopteris fragrans. The adsorption properties of twelve macroporous adsorption resins were evaluated. The three selected resins were further screened depending on the separation performance of their packed columns, in which AB-8 resin showed better separation efficiency for dryofragin and aspidin BB. In order to maximize column efficiency, the operating parameters (flow rate, ethanol concentration and volume) of the resin column chromatography were optimized and compared with the conventional resin column adsorption. After preparative separation and enrichment on resin column chromatography, the contents of dryofragin and aspidin BB in the product were 8.39- and 5.99-fold increased with recovery yields of 91.22% and 75.64%, respectively. Moreover, the regenerated adsorbent exhibited excellent reusability within at least five cycles of adsorption/desorption. It suggested that multi-targets would be enriched effectively by resin column chromatography. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：199 / 206

页数：8

共 30 条

[1]

Ai ZhiLu Ai ZhiLu, 2007, Transactions of the Chinese Society of Agricultural Engineering, V23, P245

[2]

[Anonymous], [No title captured]

[3]

Ao Z.W., 1990, FERNS HEILONGJIANG P, P121

[4] Investigation of how agitation during precipitation, and subsequent processing affects the particle size distribution and separation of α-lactalbumin enriched whey protein precipitates [J].

Byrne, EP ;

Fitzpatrick, JJ .

BIOCHEMICAL ENGINEERING JOURNAL, 2002, 10 (01) :17-25

[5]

Chang Y, 2009, North Hortic, V04, P113

[6] Optimization of luteolin separation from pigeonpea [Cajanus cajan (L.) Millsp.] leaves by macroporous resins [J].

Fu, Yujie ;

Zu, Yuangang ;

Liu, Wei ;

Efferth, Thomas ;

Zhang, Naijing ;

Liu, Xiaona ;

Kong, Yu .

JOURNAL OF CHROMATOGRAPHY A, 2006, 1137 (02) :145-152

[7]

Huang Jian-Ming, 2003, Fudan Xuebao (Yixuekexueban), V30, P267

[8]

Ito H, 1997, CHEM PHARM BULL, V45, P1720, DOI 10.1248/cpb.45.1720

[9]

Ito H, 2000, CHEM PHARM BULL, V48, P1190

[10] Anti-tumor promoting activity of Dryopteris phlorophenone derivatives [J].

Kapadia, GJ ;

Tokuda, H ;

Konoshima, T ;

Takasaki, M ;

Takayasu, J ;

Nishino, H .

CANCER LETTERS, 1996, 105 (02) :161-165

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