A neuron-specific cytoplasmic dynein isoform preferentially transports TrkB signaling endosomes

被引：84

作者：

Ha, Junghoon ^{[1
]}

Lo, Kevin W. -H. ^{[1
]}

Myers, Kenneth R. ^{[1
]}

Carr, Tiffany M. ^{[1
]}

Humsi, Michael K. ^{[1
]}

Rasoul, Bareza A. ^{[1
]}

Segal, Rosalind A. ^{[2
,3
]}

Pfister, K. Kevin ^{[1
]}

机构：

[1] Univ Virginia, Sch Med, Dept Cell Biol, Charlottesville, VA 22908 USA

[2] Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Neurobiol, Boston, MA 02115 USA

[3] Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Pediat Oncol, Boston, MA 02115 USA

来源：

JOURNAL OF CELL BIOLOGY | 2008年 / 181卷 / 06期

关键词：

D O I：

10.1083/jcb.200803150

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cytoplasmic dynein is the multisubunit motor protein for retrograde movement of diverse cargoes to microtubule minus ends. Here, we investigate the function of dynein variants, defined by different intermediate chain (IC) isoforms, by expressing fluorescent ICs in neuronal cells. Green fluorescent protein (GFP)-IC incorporates into functional dynein complexes that copurify with membranous organelles. In living PC12 cell neurites, GFP-dynein puncta travel in both the anterograde and retrograde directions. In cultured hippocampal neurons, neurotrophin receptor tyrosine kinase B (TrkB) signaling endosomes are transported by cytoplasmic dynein containing the neuron-specific IC-1B isoform and not by dynein containing the ubiquitous IC-2C isoform. Similarly, organelles containing TrkB isolated from brain by immuno-affinity puri. cation also contain dynein with IC-1 but not IC-2 isoforms. These data demonstrate that the IC isoforms de. ne dynein populations that are selectively recruited to transport distinct cargoes.

引用

页码：1027 / 1039

页数：13

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