Development of a pyrosequencing assay for rapid assessment of quinolone resistance in Acinetobacter baumannii isolates

被引：7

作者：

Deccache, Yann ^{[2
]}

Irenge, Leonid M. ^{[1
,2
]}

Savov, Encho ^{[3
]}

Ariciuc, Mihaela ^{[4
]}

Macovei, Alexandra ^{[4
]}

Trifonova, Angelina ^{[3
]}

Gergova, Ivanka ^{[3
]}

Ambroise, Jerome ^{[5
]}

Vanhoof, Raymond ^{[6
]}

Gala, Jean-Luc ^{[1
,2
]}

机构：

[1] Catholic Univ Louvain, Ctr Appl Mol Technol, Inst Expt & Clin Res, B-1200 Brussels, Belgium

[2] Belgian Armed Forces, Def Labs Dept, Brussels, Belgium

[3] Mil Med Acad Sofia, Dept Hyg & Epidemiol, Sofia, Bulgaria

[4] Bucharest Clin Emergency Hosp, Bucharest, Romania

[5] Catholic Univ Louvain, Inst Informat & Commun Technol, B-1348 Louvain, Belgium

[6] Sci Inst Publ Hlth, Unit Antibiot Res, Brussels, Belgium

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2011年 / 86卷 / 01期

关键词：

Acinetobacter baumannii; Quinolone resistance; gyrA; parC; Pyrosequencing; ANTIMICROBIAL RESISTANCE; ESCHERICHIA-COLI; PARC GENES; MUTATIONS; GYRA; MECHANISMS; EMERGENCE; STRAINS;

D O I：

10.1016/j.mimet.2011.04.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rapid and reliable assessment of Acinetobacter baumannii resistance to quinolones was successfully achieved through pyrosequencing of the gyrA and parCquinolone-resistance determining regions. A strong correlation was found between quinolone resistance and mutations in gyrA codon 83 and/or in the parC gene (codons 80 or 84). Absence of QRDR mutations was associated with susceptibility to quinolones. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：115 / 118

页数：4

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