Isolation and characterization of a cDNA encoding a papain-like cysteine protease from alfalfa

被引:1
|
作者
Yan, Longfeng [1 ,2 ]
Han, Jianguo [1 ]
Yang, Qingchuan [2 ]
Sun, Yan [1 ]
Kang, Junmei [2 ]
Liu, Zhipeng [2 ]
Wu, Mingsheng [3 ]
机构
[1] China Agr Univ, Inst Grassland Sci, Beijing 100094, Peoples R China
[2] Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100094, Peoples R China
[3] Beijing Seed Adm Stn, Beijing 100088, Peoples R China
来源
DNA SEQUENCE | 2008年 / 19卷 / 03期
关键词
abiotic stress; alfalfa (Medicago sativa L.); cysteine protease; expression;
D O I
10.1080/10253890701575166
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Protein hydrolyzation is activated and involved in response to various stress signals. In the present study, a full-length cDNA, named MsCP1, encoding a papain-like cysteine protease was obtained by degenerated primers and 3'- and 5'-RACE frorn salt-tolerant alfalfa. The cDNA contained an open reading frame encoding a deduced protein of 350 amino acids with a putative N-terminal signal peptide, NPIR vacuole-sorting signal sequence and potential N-linked glycosylation sites. The deduced sequence showed a high similarity to deduced proteins from pea, tobacco, tomato and ryegrass. Fusion expression analysis in Escherichia coli showed that the putative eukaryotic signal peptide prevented its expression in prokaryotic system. The integration and transcript of the expression elements in transgenic tobacco plants were detected with Southern blot and RT-PCR analysis.
引用
收藏
页码:274 / 281
页数:8
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