Crystallization and preliminary X-ray crystallographic studies on 4-α-glucanotransferase from Thermotoga maritima

被引：5
|
作者：
Roujeinikova, A
Raasch, C
Sedelnikova, S
Liebl, W
Rice, DW [1 ]
机构：
[1] Univ Sheffield, Dept Mol Biol & Biotechnol, Krebs Inst Biomol Res, Sheffield S10 2TN, S Yorkshire, England
[2] Univ Gottingen, Inst Mikrobiol & Genet, D-3400 Gottingen, Germany
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2001年 / 57卷
关键词：
D O I：
10.1107/S0907444901007740
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Thermotoga maritima 4-alpha -glucanotransferase (GTase), a 52 kDa molecular-weight amylolytic enzyme, has been crystallized by the hanging-drop vapour-diffusion method using PEG monomethylether 5000 as a precipitating agent. A complete data set has been collected to 2.6 Angstrom resolution using cryocooling conditions and synchrotron radiation. The crystals belong to space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 92.6, b = 180.3, c = 199.2 Angstrom.
引用
收藏
页码：1046 / 1047
页数：2
相关论文
共 50 条
  • [1] Crystallization and preliminary X-ray crystallographic studies on maltosyltransferase from Thermotoga maritima
    Burke, J
    Roujeinikova, A
    Baker, PJ
    Sedelnikova, S
    Raasch, C
    Liebl, W
    Rice, DW
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2000, 56 : 1049 - 1050
  • [2] Crystallization and preliminary X-ray crystallographic analysis of the surE protein from Thermotoga maritima
    Kwak, JE
    Ha, KS
    Lee, JY
    Im, YJ
    Park, SH
    Eom, SH
    Suh, SW
    ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2001, 57 : 612 - 613
  • [3] Overexpression, crystallization, and preliminary X-ray crystallographic analysis of shikimate dehydrogenase from Thermotoga maritima
    Lee, Hyung Ho
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 824 - 826
  • [4] Crystallization and preliminary X-ray studies of xylanase 10B from Thermotoga maritima
    Ihsanawati
    Kumasaka, T
    Kaneko, T
    Morokuma, C
    Nakamura, S
    Tanaka, N
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2003, 59 : 1659 - 1661
  • [5] Crystallization and preliminary X-ray analysis of cytosolic α-mannosidase from Thermotoga maritima
    Nakajima, M
    Fushinobu, S
    Imamura, H
    Shoun, H
    Wakagi, T
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2006, 62 : 104 - 105
  • [6] Crystallization and preliminary X-ray crystallographic analysis of CheW from Thermotoga maritima: a coupling protein of CheA and the chemotaxis receptor
    Park, SangYoun
    Crane, Brian R.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 504 - 506
  • [7] Crystallization and preliminary X-ray crystallographic characterization of TrmFO, a folate-dependent tRNA methyltransferase from Thermotoga maritima
    Cicmil, Nenad
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2008, 64 : 193 - 195
  • [8] Crystallization and preliminary X-ray characterization of a thermostable pectate lyase from Thermotoga maritima
    McDonough, MA
    Ryttersgaard, C
    Bjornvad, ME
    Lo Leggio, L
    Schülein, M
    Glad, SOS
    Larsen, S
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2002, 58 : 709 - 711
  • [9] Crystallization and preliminary X-ray analysis of Thermotoga maritima ribosome recycling factor
    Selmer, M
    Al-Karadaghi, S
    Hirokawa, G
    Kaji, A
    Liljas, A
    ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1999, 55 : 2049 - 2050
  • [10] Crystallization and preliminary X-ray studies of the protease domain of the heat-shock protein HtrA from Thermotoga maritima
    Kim, DY
    Kim, KK
    ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2002, 58 : 170 - 172
12345