Electrochemical immunoassay for the detection of stress biomarkers

被引：13

作者：

Abdulsattar, Jwan O. ^{[1
]}

Greenway, Gillian M. ^{[2
]}

Wadhawan, Jay D. ^{[3
]}

机构：

[1] Mustansiriyah Univ, Coll Sci, Dept Chem, Baghdad, Iraq

[2] Univ Hull, Dept Chem & Biochem, Cottingham Rd, Kingston Upon Hull HU6 7RX, N Humberside, England

[3] Univ Hull, Dept Chem Engn, Cottingham Rd, Kingston Upon Hull HU6 7RX, N Humberside, England

来源：

HELIYON | 2020年 / 6卷 / 03期

关键词：

Biomedical engineering; Electrochemistry; Biosensors; Immunoassay; Stress biomarkers; Point of care; CORTISOL; GOLD; IMMUNOSENSORS; REDUCTION; ELECTRODE; HORMONES;

D O I：

10.1016/j.heliyon.2020.e03558

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A rapid electrochemical immunoassay method was developed to detect and measure stress biomarkers (cortisol and cortisone) in two biological samples (Zebrafish whole-body and artificial saliva). This methodology utilizes an immunoassay approach taking advantage of the lock and key mechanism that is related to the antibody-antigen interaction depending on the reliable immobilization of the antibody labelled with ferrocene tags (Ab-Fc) on a modified tin-doped indium oxide (ITO) electrode using electrochemical instrumentation to build a POC platform. The limit of detection (LOD) obtained for this biosensor was 1.03 pg ml(-1) for cortisol and 0.68 pg ml(-1) for cortisone, respectively. The correlation coefficient was 0.9852 and 0.9841 for cortisol and cortisone, respectively with a linear concentration from (0-50 ng ml(-1)) which covers the standard levels of stress hormones in both selected biological samples. The incubation time was investigated and 30 min was found to be the optimum incubation time. This time would be acceptable for the POC system as total process time can be determined within 35 min.

引用

页数：7

共 41 条

[1] A sensitive chemiluminescence based immunoassay for the detection of cortisol and cortisone as stress biomarkers [J].

Abdulsattar, Jwan O. ;

Greenway, Gillian M. .

JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY, 2019, 10 (01)

[2] Antibody functionalized interdigitated μ-electrode (IDμE) based impedimetric cortisol biosensor [J].

Arya, Sunil K. ;

Chornokur, Ganna ;

Venugopal, Manju ;

Bhansali, Shekhar .

ANALYST, 2010, 135 (08) :1941-1946

[3]

Canavello PR, 2011, NEUROMETHODS, V51, P135, DOI 10.1007/978-1-60761-953-6_11

[4]

Conrad CD, 2008, REV NEUROSCIENCE, V19, P395

[5]

Darwish Ibrahim A, 2006, Int J Biomed Sci, V2, P217

[6] Voltammetric Immunoassay for the Detection of Protein Biomarkers [J].

Dou, Yue-Hua ;

Haswell, Stephen J. ;

Greenman, John ;

Wadhawan, Jay .

ELECTROANALYSIS, 2012, 24 (02) :264-272

[7] The Relationship between Cortisol Activity during Cognitive Task and Posttraumatic Stress Symptom Clusters [J].

Duan, Hongxia ;

Wang, Li ;

Zhang, Liang ;

Liu, Jing ;

Zhang, Kan ;

Wu, Jianhui .

PLOS ONE, 2015, 10 (12)

[8] A Comparative Study of Electrochemical Reduction of 4-Nitrophenyl Covalently Grafted on Gold and Carbon [J].

Gui, Alicia L. ;

Liu, Guozhen ;

Chockalingam, Muthukumar ;

Le Saux, Guillaume ;

Luais, Erwann ;

Harper, Jason B. ;

Gooding, J. Justin .

ELECTROANALYSIS, 2010, 22 (16) :1824-1830

[9]

Guilliams TG., 2010, Stnd, V9, P1

[10] Traumatic memories, post-traumatic stress disorder and serum cortisol levels in long-term survivors of the acute respiratory distress syndrome [J].

Hauer, Daniela ;

Weis, Florian ;

Krauseneck, Till ;

Vogeser, Michael ;

Schelling, Gustav ;

Roozendaal, Benno .

BRAIN RESEARCH, 2009, 1293 :114-120

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