CYCLOOXYGENASE AND NITRIC OXIDE SYNTHASE IN THE PRESYMPATHETIC NEURONS IN THE PARAVENTRICULAR HYPOTHALAMIC NUCLEUS ARE INVOLVED IN RESTRAINT STRESS-INDUCED SYMPATHETIC ACTIVATION IN RATS

被引:29
作者
Yamaguchi, N. [1 ]
Ogawa, S. [1 ]
Okada, S. [2 ]
机构
[1] Univ Tsukuba, Grad Sch Comprehens Human Sci, Lab Behav Neuroendocrinol, Tsukuba, Ibaraki 3058577, Japan
[2] Kochi Univ, Grad Sch Med, Dept Pharmacol, Nanko Ku, Kochi 7838505, Japan
基金
日本学术振兴会;
关键词
stress; COX; NOS; sympathetic nervous system; retrograde tracing; CORTICOTROPIN-RELEASING-FACTOR; IMMOBILIZATION STRESS; MESSENGER-RNA; SPINAL-CORD; CARDIOVASCULAR-RESPONSES; PROSTAGLANDIN SYNTHESIS; BIOCHEMICAL-ALTERATIONS; OVARIECTOMIZED RATS; BLOOD-PRESSURE; BRAIN;
D O I
10.1016/j.neuroscience.2010.07.051
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Stress is one of the important factors to activate the sympathetic nervous system. We recently reported that central administration of corticotropin-releasing factor (CRF), known as a stress-related neuropeptide, increases the expression of both cyclooxygenase (COX) and nitric oxide synthase (NOS) in presympathetic neurons in the paraventricular hypothalamic nucleus (PVN). In the present study, therefore, we investigated whether brain COX and NOS can also mediate restraint stress (RS)-induced sympathetic activation by assessing the plasma catecholamine levels and neuronal activation of presympathetic neurons in the PVN. In addition, we examined effects of RS on the expression of both COX and NOS isozymes in the presympathetic PVN neurons. Intraperitoneal administration of an inhibitor for COX-1, COX-2 or inducible NOS (iNOS), but not for neuronal NOS (nNOS), reduced RS-induced elevation of plasma catecholamine levels and Fos expression in the presympathetic PVN neurons. Moreover, RS increased the expression of COX-1, COX-2 and iNOS in the presympathetic PVN neurons, whereas nNOS expression did not change. These results suggest that COX-1, COX-2 and iNOS in the presympathetic PVN neurons mediate acute RS-induced sympathetic activation. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:773 / 781
页数:9
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