The immunophilin FKBP12 functions as a common inhibitor of the TGF beta family type I receptors

被引：319

作者：

Wang, TW ^{[1
]}

Li, BY ^{[1
]}

Danielson, PD ^{[1
]}

Shah, PC ^{[1
]}

Rockwell, S ^{[1
]}

Lechleider, RJ ^{[1
]}

Martin, J ^{[1
]}

Manganaro, T ^{[1
]}

Donahoe, PK ^{[1
]}

机构：

[1] NCI,CHEMOPREVENT LAB,BETHESDA,MD 20892

来源：

CELL | 1996年 / 86卷 / 03期

关键词：

D O I：

10.1016/S0092-8674(00)80116-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The immunophilin FKBP12 is an evolutionarily conserved abundant protein; however, its physiological roles remain poorly defined. Here we report that FKBP12 is a common cytoplasmic interactor of TGF beta family type I receptors. FKBP12 binds to ligand-free TGF beta type I receptor, from which it is released upon a ligand-induced, type II receptor mediated phosphorylation of the type I receptor. Blocking FKBP12/type I receptor interaction with FK506 nonfunctional derivatives enhances the ligand activity, indicating that FKBP12 binding is inhibitory to the signaling pathways of the TGF beta family ligands. Overexpression of a myristylated FKBP12 in Mv1Lu cell specifically inhibits two separate pathways activated by TGF beta, and two point mutations on FKBP12 (G89P, 190K) abolish the inhibitory activity of FKBP12, suggesting that FKBP12 may dock a cytoplasmic protein to the type I receptors to inhibit TGF beta family mediated signaling.

引用

页码：435 / 444

页数：10

共 47 条

[1] TGF-BETA RECEPTORS AND ACTIONS [J].