Genetic Diversity and Post-Translational Modification Analysis of L-Asparaginase in Six Strains of Sordaria fimicola

Filamentous fungi are a preferable source of production of L-asparaginase as compare to other organisms In the present study L-asparaginase gene NUC05624, which encode its production was amplified and sequenced to assess the genetic diversity and post-translational modifications (PTMs). A comparison was made among six different strains of filamentous fungus Sordaria fimicola with reference species Neurospora crassa. During this study, we calculated single nucleotide polymorphism (SNP) in the exonic region of the L-asparaginase gene in six parental strains (S1, S2, S3 N5 N6 and N7) of S. fimicola. SNP(s) on seven different nucleotides i.e. G(158)C; T(256)A; A(715)T; T(936)A; G(1026)C; C(1137)G; T(1301)A with 50% of percentage prevalence was observed in Si S2 S3 strains of S.,fimicola, isolated from the S-slope of EC. Genetic polymorphism on position T(559)G and A(1665)C with 50% of percentage prevalence was calculated in N5, N6 and N7 strains only not in any strain of S slope. PTMs were predicted by using various online bioinformatics tools both in S. fimicola and reference species. The molecular and post-translational modification data in this study revealed that environmental stresses affected specific genes by SNP, which may result in genomic diversity among the organisms and their proteins. (C) 2020 Friends Science Publishers