Acyl chain length affects ceramide action on sterol/sphingomyelin-rich domains

被引:59
作者
Nybond, S [1 ]
Björkqvist, YJE [1 ]
Ramstedt, B [1 ]
Slotte, JP [1 ]
机构
[1] Abo Akad Univ, Dept Biochem & Pharm, FI-20520 Turku, Finland
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2005年 / 1718卷 / 1-2期
基金
芬兰科学院;
关键词
sterol partitioning; cholestatrienol; trans-parinaric acid; quenching; 7-doxyl phosphatidylcholine;
D O I
10.1016/j.bbamem.2005.10.009
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effects of ceramides with varying saturated N-linked acyl chains (C2-C14) on cholesterol displacement from sphingomyelin-rich domains and on the stability of ordered domains were studied. The bilayers examined were made from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), D-erythro-N-pahnitoyi-sphingomyelin (PSM), D-erythro-N-acyl-sphingosine, and cholesterol (60:15:15:10 mol%, respectively). Cholestatrienol (CTL) or D-erythro-N-trans-parinoyl-spbingomyelin (tParSM) were used as reporter molecules (at I mol%) for the ordered domains, and 1-palmitoyl-2-stearoyl-(7-doxyl)-sn-glycero-3-phosphocholine (7SLPC) as a fluorescence quencher (30 mol%, replacing POPC) in the liquid-disordered phase. The results indicate that the ceramide had to have an N-linked acyl chain with at least 8 methylene units in order for it to displace cholesterol from the sphingomyelin-rich domains at the concentration used. The melting of the sphingomyelin-rich domain shifted to higher temperatures (compared to the ceramide-free control) with C2, C12 and longer chain ceramides, whereas C4-C10 ceramides led to domain melting at lower temperatures than control. This study shows that short-chain ceramides do not have the same effects on sterol- and sphingomyelin-rich domains as naturally occurring longer-chain ceramides have. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:61 / 66
页数:6
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