Differentiation elicits negative regulation of human β-galactoside α2,6-sialyltransferase at the mRNA level in the HL-60 cell line

被引：25

作者：

Taniguchi, A ^{[1
]}

Higai, K ^{[1
]}

Hasegawa, Y ^{[1
]}

Utsumi, K ^{[1
]}

Matsumoto, K ^{[1
]}

机构：

[1] Toho Univ, Sch Pharmaceut Sci, Dept Clin Chem, Chiba 2748510, Japan

来源：

FEBS LETTERS | 1998年 / 441卷 / 02期

关键词：

sialyltransferase; HL-60; cell; cell differentiation; gene expression; Sambucus nigra agglutinin; alternative splicing;

D O I：

10.1016/S0014-5793(98)01548-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We studied the regulation of the beta-galactoside alpha 2,6-sialyltransferase (hST6Gal I) gene during HL-60 cell differentiation induced with dimethyl sulfoxide (DMSO), all transretinoic acid (ATRA), and phorbol myristate acetate (PMA), During HL-60 cell line differentiation, cell surface levels of alpha 2,6-sialic acids expression decreased, as measured by flow cytometric analysis using Sambucus nigra a agglutinin (SNA), Activities of hST6Gal I and levels of hST6Gal I mRNA dramatically decreased after 1 day of stimulation. Using reverse transcription polymerase chain reaction (PT-PCR), we found the major hST6Gal I mRNA isoform in HL-60 cells contains 5'-untranslated exons Y and Z, These results suggest that the expression of cell surface alpha 2,6-sialic acids is controlled at the mRNA level, which is regulated by a promoter located 5'-upstream of exon Y. (C) 1998 Federation of European Biochemical Societies.

引用

页码：191 / 194

页数：4

共 33 条

[1] CHARACTERIZATION OF A PROMOTER REGION SUPPORTING TRANSCRIPTION OF A NOVEL HUMAN BETA-GALACTOSIDE ALPHA-2,6-SIALYLTRANSFERASE TRANSCRIPT IN HEPG2 CELLS [J].

AASENG, DA ;

ASHEIM, HC ;

DEGGERDAL, A ;

SMELAND, E ;

FUNDERUD, S .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1995, 1261 (01) :166-169

[2] CELL-SPECIFIC EXPRESSION OF HUMAN BETA-GALACTOSIDE ALPHA-2,6-SIALYTRANSFERASE TRANSCRIPTS DIFFERING IN THE 5' UNTRANSLATED REGION [J].

AASHEIM, HC ;

AASENG, DA ;

DEGGERDAL, A ;

BLOMHOFF, HK ;

FUNDERUD, S ;

SMELAND, EB .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 213 (01) :467-475

[3] CARBOHYDRATE LIGANDS OF THE LEC CELL-ADHESION MOLECULES [J].

BRANDLEY, BK ;

SWIEDLER, SJ ;

ROBBINS, PW .

CELL, 1990, 63 (05) :861-863

[4] alpha 1,3-L-Fucosyltransferase expression in developing human myeloid cells Antigenic, enzymatic, and mRNA analyses [J].

Clarke, JL ;

Watkins, WM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (17) :10317-10328

[5] THE EXPRESSION OF SOLUBLE AND CELL-BOUND ALPHA-2,6 SIALYLTRANSFERASE IN HUMAN COLONIC-CARCINOMA CACO-2 CELLS CORRELATES WITH THE DEGREE OF ENTEROCYTIC DIFFERENTIATION [J].

DALLOLIO, F ;

MALAGOLINI, N ;

SERAFINICESSI, F .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 184 (03) :1405-1410

[6] Differentiation-dependent expression of human beta-galactoside alpha 2,6-sialyltransferase mRNA in colon carcinoma CaCo-2 cells [J].

DallOlio, F ;

Malagolini, N ;

Guerrini, S ;

Lau, JTY ;

SerafiniCessi, F .

GLYCOCONJUGATE JOURNAL, 1996, 13 (01) :115-121

[7] MEMBRANE DIFFERENTIATION IN HUMAN MYELOID CELLS - EXPRESSION OF UNIQUE PROFILES OF CELL-SURFACE GLYCOPROTEINS IN MYELOID LEUKEMIC-CELL LINES BLOCKED AT DIFFERENT STAGES OF DIFFERENTIATION AND MATURATION [J].

FUKUDA, M ;

KOEFFLER, HP ;

MINOWADA, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (10) :6299-6303

[8] SPECIFIC CHANGES IN THE SURFACE GLYCOPROTEIN PATTERN OF HUMAN PROMYELOCYTIC LEUKEMIC-CELL LINE HL-60 DURING MORPHOLOGIC AND FUNCTIONAL-DIFFERENTIATION [J].

GAHMBERG, CG ;

NILSSON, K ;

ANDERSSON, LC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (08) :4087-4091

[9] Differential gene expression of a human α2,3-sialyltransferase in leukaemic cell lines and leucocytes [J].

Gassen, U ;

Kelm, S ;

Schauer, R .

FEBS LETTERS, 1998, 427 (01) :91-95

[10]

HANASAKI K, 1994, J BIOL CHEM, V269, P10637

← 1 2 3 4 →