DNA methylation for cervical cancer screening: a training set in China

被引:41
作者
Kong, Linghua [1 ]
Wang, Linhai [2 ]
Wang, Ziyun [2 ]
Xiao, Xiaoping [1 ]
You, Yan [3 ]
Wu, Huanwen [3 ]
Wu, Ming [1 ]
Liu, Pei [2 ]
Li, Lei [1 ]
机构
[1] Peking Union Med Coll Hosp, Dept Obstet & Gynecol, Shuaifuyuan 1, Beijing 100730, Peoples R China
[2] Beijing SinoMDgene Technol Co Ltd, Floor 3,Bldg 14,Guo Sheng Sci Pk,1 Kangding St, Beijing 100176, Peoples R China
[3] Peking Union Med Coll Hosp, Dept Pathol, Beijing 100730, Peoples R China
关键词
Cervical cancer; Cervical intraepithelial neoplasia; DNA methylation; High-risk human papillomavirus; Cytology; Training set; DIFFERENT HISTOLOGICAL SUBTYPES; HUMAN-PAPILLOMAVIRUS; TRIAGE TEST; RISK; ADENOCARCINOMA; HYPERMETHYLATION; PREVALENCE; PRECANCER; LESIONS; STATISTICS;
D O I
10.1186/s13148-020-00885-7
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background Despite rapid improvements in DNA methylation tools for cervical cancer screening, few robust, exploratory studies have been performed using the combination of two host genes,EPB41L3andJAM3, newly developed assays. Methods A review of abnormal liquid-based cytology and/or high-risk human papillomavirus (hrHPV) data from outpatient clinics in the study center from March 2018 to March 2019 was performed. Eligible patients with definitive histological pathology results were included, and their residual cytology samples were assessed forEPB41L3andJAM3methylation. The diagnostic accuracies of various screening strategies for definitive pathology and for cervical intraepithelial neoplasia (CIN) 2 or more severe lesions (CIN2+) were compared. Results In total, 306 patients were successfully tested; 301 cases with cervical histological pathology were included in the final analysis, including 118 (39.2%) and 183 (60.8%) cases of inflammation/CIN1 and CIN2+, respectively. Regarding CIN2+ detection, methylation status and hrHPV plus methylation had similar positive predictive values (0.930 and 0.954, respectively,p= 0.395). Additionally, hrHPV, methylation, and hrHPV plus methylation had similar negative predictive values (0.612, 0.679, and 0.655,p= 0.677) that were significantly higher than that of cytology alone (0.250,pvalues 0.012, 0.001, and 0.001, respectively). For 49 cases with negative hrHPV results, positive methylation alone was able to differentiate CIN2+ from inflammation/CIN1. Conclusions Methylation of bothEPB41L3andJAM3is an accurate and feasible screening method for CIN2+.
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