Two-photon excited hemoglobin fluorescence

被引：118

作者：

Zheng, Wei ^{[1
]}

Li, Dong ^{[1
]}

Zeng, Yan ^{[1
]}

Luo, Yi ^{[2
]}

Qu, Jianan Y. ^{[1
]}

机构：

[1] Hong Kong Univ Sci & Technol, Dept Elect & Comp Engn, Biomed Engn Program, Hong Kong, Hong Kong, Peoples R China

[2] Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Bio X Div, Hefei 230026, Anhui, Peoples R China

来源：

BIOMEDICAL OPTICS EXPRESS | 2011年 / 2卷 / 01期

关键词：

IN-VIVO; EMISSION MICROSCOPY; EXCITATION; GENERATION; DYNAMICS;

D O I：

10.1364/BOE.2.000071

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We discovered that hemoglobin emits high energy Soret fluorescence when two-photon excited by the visible femtosecond light sources. The unique spectral and temporal characteristics of hemoglobin fluorescence were measured by using a time-resolved spectroscopic detection system. The high energy Soret fluorescence of hemoglobin shows the spectral peak at 438 nm with extremely short lifetime. This discovery enables two-photon excitation fluorescence microscopy to become a potentially powerful tool for in vivo label-free imaging of blood cells and vessels. (C)2010 Optical Society of America

引用

页码：71 / 79

页数：9

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