Evaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli

被引：39

作者：

Ellila, Simo ^{[1
]}

Jurvansuu, Jaana Maria ^{[1
]}

Iwai, Hideo ^{[1
]}

机构：

[1] Univ Helsinki, Inst Biotechnol, Res Program Struct Biol & Biophys, FIN-00014 Helsinki, Finland

来源：

FEBS LETTERS | 2011年 / 585卷 / 21期

基金：

芬兰科学院;

关键词：

Protein splicing; Trans-splicing; Segmental labeling; Protein ligation; Intein; DNAE INTEIN; MULTIDOMAIN PROTEIN; NOSTOC-PUNCTIFORME; SPLIT INTEINS; IN-VIVO; GLUTAMINE; LIGATION; DESIGN; TRANS;

D O I：

10.1016/j.febslet.2011.10.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein splicing catalyzed by inteins has enabled various biotechnological applications such as protein ligation. Successful applications of inteins are often limited by splicing efficiency. Here, we report the comparison of protein splicing between 20 different inteins from various organisms in identical contexts to identify robust inteins with foreign exteins. We found that RadA intein from Pyrococcus horikoshii and an engineered DnaB intein from Nostoc punctiforme demonstrated an equally efficient splicing activity to the previously reported highly efficient DnaE intein from Nostoc punctiforme. The newly identified inteins with efficient cis-splicing activity can be good starting points for the further development of new protein engineering tools. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：3471 / 3477

页数：7

共 24 条

[1] Protein splicing of inteins with atypical glutamine and aspartate c-terminal residues [J].

Amitai, G ;

Dassa, B ;

Pietrokovski, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (05) :3121-3131

[2] In Vivo and In Vitro Protein Ligation by Naturally Occurring and Engineered Split DnaE Inteins [J].

Aranko, A. Sesilja ;

Zuger, Sara ;

Buchinger, Edith ;

Iwai, Hideo .

PLOS ONE, 2009, 4 (04)

[3] Segmental Isotopic Labeling of a Central Domain in a Multidomain Protein by Protein Trans-Splicing Using Only One Robust DnaE Intein [J].

Busche, Alena E. L. ;

Aranko, A. Sesija ;

Talebzadeh-Farooji, Mehdi ;

Bernhard, Frank ;

Doetsch, Volker ;

Iwai, Hideo .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (33) :6128-6131

[4] Protein trans-splicing and characterization of a split family B-type DNA polymerase from the hyperthermophilic archaeal parasite Nanoarchaeum equitans [J].

Choi, JJ ;

Nam, KH ;

Min, B ;

Kim, SJ ;

Söll, D ;

Kwon, ST .

JOURNAL OF MOLECULAR BIOLOGY, 2006, 356 (05) :1093-1106

[5] Trans protein splicing of cyanobacterial split inteins in endogenous and exogenous combinations [J].

Dassa, Bareket ;

Amitai, Gil ;

Caspi, Jonathan ;

Schueler-Furman, Ora ;

Pietrokovski, Shmuel .

BIOCHEMISTRY, 2007, 46 (01) :322-330

[6] NMR resonance assignment of DnaE intein from Nostoc punctiforme [J].

Heinamaki, Kimmo ;

Oeemig, Jesper S. ;

Djupsjobacka, Janica ;

Iwai, Hideo .

BIOMOLECULAR NMR ASSIGNMENTS, 2009, 3 (01) :41-43

[7] Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme [J].

Iwai, H ;

Züger, S ;

Jin, J ;

Tam, PH .

FEBS LETTERS, 2006, 580 (07) :1853-1858

[8] Cyclic green fluorescent protein produced in vivo using an artificially split PI-PfuI intein from Pyrococcus furiosus [J].

Iwai, H ;

Lingel, A ;

Plückthun, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (19) :16548-16554

[9] Protein splicing of a Pyrococcus abyssi intein with a C-terminal glutamine [J].

Mills, KV ;

Manning, JS ;

Garcia, AM ;

Wuerdeman, LA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (20) :20685-20691

[10] Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro [J].

Muona, Mikko ;

Aranko, A. Sesilja ;

Raulinaitis, Vytas ;

Iwai, Hideo .

NATURE PROTOCOLS, 2010, 5 (03) :574-587

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