Microbial biosensors

被引:441
作者
D'Souza, SF [1 ]
机构
[1] Bhabha Atom Res Ctr, Nucl Agr & Biotechnol Div, Bombay 400085, Maharashtra, India
关键词
microbial biosensors; immobilised cells; bioluminescence biosensors (cells); permeabilised cells; environmental biosensors; food biosensors;
D O I
10.1016/S0956-5663(01)00125-7
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A microbial biosensor consists of a transducer in conjunction with immobilised viable or non-viable microbial cells. Non-viable cells obtained after permeabilisation or whole cells containing periplasmic enzymes have mostly been used as an economical substitute for enzymes. Viable cells make use of the respiratory and metabolic functions of the cell, the analyte to be monitored being either a substrate or an inhibitor of these processes. Bioluminescence-based microbial biosensors have also been developed using genetically engineered microorganisms constructed by fusing the lux gene with an inducible gene promoter for toxicity and bioavailability testing. In this review, some of the recent trends in microbial biosensors with reference to the advantages and limitations are been discussed. Some of the recent applications of microbial biosensors in environmental monitoring and for use in food, fermentation and allied fields have been reviewed. Prospective future microbial biosensor designs have also been identified. (C) 2001 Elsevier Science BY. All rights reserved.
引用
收藏
页码:337 / 353
页数:17
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