Identification of temperature stress-induced differentially expressed genes of tall fescue leaves

In the present study, we investigated temperature stress-responsible differentially expressed genes (DEGs) using annealing control primer (ACP)-based differentially display reverse transcription-polymerase chain reaction technique in tall fescue leaves. Using 120 ACPs set, 10 genes were up or down regulated under cold or heat stresses followed by sequencing to investigate functional information. In cold stress, each three DEGs were up and down regulated respectively. In the meantime 6 DEGs were up regulated and 2 DEGs were down regulated under heat stress. Of these DEGs, pathogen related protein10-1, iron-sulfur assembly protein lacA, ABC transporter C family member 14, lipoxygenase 11, protein cornichon-like protein and uncharacterized protein LOC100823022 were identified. These up-regulated genes may play a pivotal role in effective resistance mechanism under temperature stress in tall fescue.