Molecular scale imaging of F-actin assemblies immobilized on a photopolymer surface

被引：32

作者：

Ikawa, Taiji

Hoshino, Fumihiko

Watanabe, Osamu

Li, Youli ^{[1
]}

Pincus, Philip

Safinya, Cyrus R.

机构：

[1] Toyota Cent Res & Dev Labs Inc, Nagakute, Aichi 4801192, Japan

[2] Univ Calif Santa Barbara, Mat Res Lab, Dept Mat, Santa Barbara, CA 93106 USA

[3] Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

[4] Univ Calif Santa Barbara, Dept Mol & Cellular, Santa Barbara, CA 93106 USA

[5] Univ Calif Santa Barbara, Dept Dev Biol, Santa Barbara, CA 93106 USA

来源：

PHYSICAL REVIEW LETTERS | 2007年 / 98卷 / 01期

关键词：

D O I：

10.1103/PhysRevLett.98.018101

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

A photo-immobilization based process is presented for direct imaging of hierarchical assemblies of biopolymers using atomic force microscopy (AFM). The technique was used to investigate the phase behavior of F-actin aggregates as a function of concentration of the divalent cation Mg2+. The data provided direct experimental evidence of a coil-on-coil (braided) structure of F-actin bundles formed at high Mg2+ concentrations. At intermediate Mg2+ concentrations, the data showed the first images of the two-dimensional nematic rafts discovered by recent x-ray studies and theoretical treatments.

引用

页数：4

共 23 条

[1] THE 3-DIMENSIONAL STRUCTURE OF THE ACTIN FILAMENT REVISITED [J].