Comparison of liquid chromatography-mass spectrometry and direct infusion microchip electrospray ionization mass spectrometry in global metabolomics of cell samples

被引：7
作者：
Poho, Paivi [1 ,2 ]
Lipponen, Katriina [1 ,2 ]
Bespalov, Maxim M. [3 ]
Sikanen, Tiina [1 ,2 ]
Kotiaho, Tapio [1 ,2 ,4 ]
Kostiainen, Risto [1 ,2 ]
机构：
[1] Univ Helsinki, Fac Pharm, Drug Res Program, FI-00014 Helsinki, Finland
[2] Univ Helsinki, Fac Pharm, Div Pharmaceut Chem & Technol, FI-00014 Helsinki, Finland
[3] Univ Helsinki, Fac Med, Biomed Stem Cell Ctr, Stem Cells & Metab Res Program, FI-00014 Helsinki, Finland
[4] Univ Helsinki, Fac Sci, Dept Chem, FI-00014 Helsinki, Finland
来源：
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES | 2019年 / 138卷
基金：
欧洲研究理事会;
关键词：
Metabolomics; Electrospray; Microchip; Liquid chromatography-mass spectrometry; Cells; Direct infusion mass spectrometry; STEM-CELLS; MS; DISCOVERY;
D O I：
10.1016/j.ejps.2019.104991
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
In this study, the feasibility of direct infusion electrospray ionization microchip mass spectrometry (chip-MS) was compared to the commonly used liquid chromatography-mass spectrometry (LC-MS) in non-targeted metabolomics analysis of human foreskin fibroblasts (HFF) and human induced pluripotent stem cells (hiPSC) reprogrammed from HFF. The total number of the detected features with chip-MS and LC-MS were 619 and 1959, respectively. Approximately 25% of detected features showed statistically significant changes between the cell lines with both analytical methods. The results show that chip-MS is a rapid and simple method that allows high sample throughput from small sample volumes and can detect the main metabolites and classify cells based on their metabolic profiles. However, the selectivity of chip-MS is limited compared to LC-MS and chip-MS may suffer from ion suppression.
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收藏
页数：7
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